Effect of benomyl on Saccharomyces cerevisiae during continuous cultivation.

The effect of Benomyl on Saccharomyces cerevisiae during continuous cultivation was investigated. The RNA, total protein and phosphorous contents decreased while the trehalose content increased with increasing Benomyl concentrations when an RD mutant and the parent strain were cultivated at different Benomyl concentrations under continuous conditions. The specific oxygen uptake rate of the respiratory deficient mutant (RD mutant) was approximately 5 times lower in comparison to the parent strain. The RQ value for the RD mutant was higher than 1 which clearly indicated the anaerobic metabolism of glucose degradation. The RD mutant lacked cytochrome aa3, while the cytochromes a and b contents were lower than those of the parent strain.