Literature DB >> 946822

Compensator transducer increases ease, accuracy, and rapidity of measuring changes in specimen birefringence with polarization microscopy.

E D Salmon, G W Ellis.   

Abstract

An instrument has been designed to improve substantially the efficiency and convenience of measuring specimen birefringence retardation (BR) with a conventional Brace-Köhler compensator. The design is based on the precise transduction of the angular position of the compensator's Vernier dial to an easily visible red-illuminated display on a digital voltmeter. The instrument display is accurate to within 0.1 degrees over a range of +/-25 degrees in the compensator vernier dial position which is similar to the precision obtainable with an ordinary vernier and the Brace-Köhler method of measuring BR of biological specimens. For convenience in data reduction the display can also be read directly in nanometre units, with a similar precision for specimen BR in the range of 5 nm or less which is typical of birefringent fine structures in living cells.

Entities:  

Mesh:

Year:  1976        PMID: 946822     DOI: 10.1111/j.1365-2818.1976.tb02384.x

Source DB:  PubMed          Journal:  J Microsc        ISSN: 0022-2720            Impact factor:   1.758


  5 in total

1.  Functional implications of cold-stable microtubules in kinetochore fibers of insect spermatocytes during anaphase.

Authors:  E D Salmon; D A Begg
Journal:  J Cell Biol       Date:  1980-06       Impact factor: 10.539

2.  Control mechanisms of the cell cycle: role of the spatial arrangement of spindle components in the timing of mitotic events.

Authors:  G Sluder; D A Begg
Journal:  J Cell Biol       Date:  1983-09       Impact factor: 10.539

3.  Quantitative studies on the polarization optical properties of living cells. I. Microphotometric birefringence detection system.

Authors:  Y Hiramoto; Y Hamaguchi; Y Shôji; S Shimoda
Journal:  J Cell Biol       Date:  1981-04       Impact factor: 10.539

4.  Rapid rate of tubulin dissociation from microtubules in the mitotic spindle in vivo measured by blocking polymerization with colchicine.

Authors:  E D Salmon; M McKeel; T Hays
Journal:  J Cell Biol       Date:  1984-09       Impact factor: 10.539

5.  Calcium-labile mitotic spindles isolated from sea urchin eggs (Lytechinus variegatus).

Authors:  E D Salmon; R R Segall
Journal:  J Cell Biol       Date:  1980-08       Impact factor: 10.539

  5 in total

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