Literature DB >> 9464

A stable, radioactive substrate emulsion for assay of lipoprotein lipase.

P Nilsson-Ehle, M C Schotz.   

Abstract

A method is described for the assay of lipoprotein lipase, using a stable, radioactive substrate emulsion. Fatty acid-labeled trioleoylglycerol was emulsified by homogenization in glycerol with lecithin as detergent. This anhydrous emulsion was stable for at least six weeks. Substrate solutions for enzyme assay were prepared by diluting the emulsion with buffer containing serum and albumin. The fatty acid produced on hydrolysis was isolated in a one-step liquid-liquid partition system. Incubations with extracts of acetone powders from adipose tissue displayed characteristics of lipoprotein lipase activity, i.e., serum dependence and inhibition by NaCl and protamine. The method is rapid (less than 1 hour), sensitive and reproducible, and suitable for routine use.

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Year:  1976        PMID： 9464

Source DB:  PubMed          Journal:  J Lipid Res        ISSN： 0022-2275            Impact factor:   5.922


  145 in total

1.  Effect of conjugated linoleic acid on body composition in mice.

Authors:  Y Park; K J Albright; W Liu; J M Storkson; M E Cook; M W Pariza
Journal:  Lipids       Date:  1997-08       Impact factor: 1.880

2.  Determination of lipoprotein lipase activity using a novel fluorescent lipase assay.

Authors:  Debapriya Basu; Jahan Manjur; Weijun Jin
Journal:  J Lipid Res       Date:  2011-01-26       Impact factor: 5.922

3.  Scavenger receptor CD36 mediates uptake of high density lipoproteins in mice and by cultured cells.

Authors:  May Brundert; Joerg Heeren; Martin Merkel; Antonella Carambia; Johannes Herkel; Peter Groitl; Thomas Dobner; Rajasekhar Ramakrishnan; Kathryn J Moore; Franz Rinninger
Journal:  J Lipid Res       Date:  2011-01-09       Impact factor: 5.922

4.  Brefeldin A enables synthesis of active lipoprotein lipase in cld/cld and castanospermine-treated mouse brown adipocytes via translocation of Golgi components to endoplasmic reticulum.

Authors:  J W Park; E J Blanchette-Mackie; R O Scow
Journal:  Biochem J       Date:  1996-07-01       Impact factor: 3.857

5.  Recombinant human cachectin/tumor necrosis factor but not interleukin-1 alpha downregulates lipoprotein lipase gene expression at the transcriptional level in mouse 3T3-L1 adipocytes.

Authors:  R Zechner; T C Newman; B Sherry; A Cerami; J L Breslow
Journal:  Mol Cell Biol       Date:  1988-06       Impact factor: 4.272

6.  Effect of chronic intermittent hypoxia on triglyceride uptake in different tissues.

Authors:  Qiaoling Yao; Mi-Kyung Shin; Jonathan C Jun; Karen L Hernandez; Neil R Aggarwal; Jason R Mock; Jason Gay; Luciano F Drager; Vsevolod Y Polotsky
Journal:  J Lipid Res       Date:  2013-02-05       Impact factor: 5.922

7.  Rare loss-of-function mutations in ANGPTL family members contribute to plasma triglyceride levels in humans.

Authors:  Stefano Romeo; Wu Yin; Julia Kozlitina; Len A Pennacchio; Eric Boerwinkle; Helen H Hobbs; Jonathan C Cohen
Journal:  J Clin Invest       Date:  2008-12-15       Impact factor: 14.808

8.  Enteral feeding a structured lipid emulsion containing fish oil prevents the fatty liver of sepsis.

Authors:  S Lanza-Jacoby; H Phetteplace; R Tripp
Journal:  Lipids       Date:  1995-08       Impact factor: 1.880

9.  Prostaglandins inhibit lipoprotein lipase gene expression in macrophages.

Authors:  J B Desanctis; L Varesio; D Radzioch
Journal:  Immunology       Date:  1994-04       Impact factor: 7.397

10.  Lipoprotein lipase expression exclusively in liver. A mouse model for metabolism in the neonatal period and during cachexia.

Authors:  M Merkel; P H Weinstock; T Chajek-Shaul; H Radner; B Yin; J L Breslow; I J Goldberg
Journal:  J Clin Invest       Date:  1998-09-01       Impact factor: 14.808
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