OBJECTIVE: This study examined the effects of peroxynitrite (ONOO-) on cardiac function and cellular injury following ischemia (30 min) and reperfusion (60 min) in isolated perfused rat hearts. METHODS: 3-Morpholinosydnonimine (SIN-1, 0.1 mM), an ONOO- donor, was administered alone or combined with superoxide dismutase (SOD, 300 U/ml) or glutathione (GSH, 1 mM) at the time of reperfusion. RESULTS: Administration of SIN-1 alone significantly aggravated post-ischemic myocardial injury characterized by depressed cardiac function recovery (p < 0.05 vs. vehicle), increased lactic dehydrogenase (LDH) and creatine kinase (CK) release (p < 0.01 vs. vehicle), and enlarged necrotic size (p < 0.01 vs. vehicle). The co-administration of either SOD to decrease the formation of ONOO-, or GSH to increase the detoxification of ONOO-, completely blocked the detrimental effects of SIN-1 and exerted significant cardioprotective effects against reperfusion injury. CONCLUSION: These results suggest that ONOO- may play a significant role in postischemic myocardial injury.
OBJECTIVE: This study examined the effects of peroxynitrite (ONOO-) on cardiac function and cellular injury following ischemia (30 min) and reperfusion (60 min) in isolated perfused rat hearts. METHODS:3-Morpholinosydnonimine (SIN-1, 0.1 mM), an ONOO-donor, was administered alone or combined with superoxide dismutase (SOD, 300 U/ml) or glutathione (GSH, 1 mM) at the time of reperfusion. RESULTS: Administration of SIN-1 alone significantly aggravated post-ischemic myocardial injury characterized by depressed cardiac function recovery (p < 0.05 vs. vehicle), increased lactic dehydrogenase (LDH) and creatine kinase (CK) release (p < 0.01 vs. vehicle), and enlarged necrotic size (p < 0.01 vs. vehicle). The co-administration of either SOD to decrease the formation of ONOO-, or GSH to increase the detoxification of ONOO-, completely blocked the detrimental effects of SIN-1 and exerted significant cardioprotective effects against reperfusion injury. CONCLUSION: These results suggest that ONOO- may play a significant role in postischemic myocardial injury.
Authors: Mark J Kohr; Honglan Wang; Debra G Wheeler; Murugesan Velayutham; Jay L Zweier; Mark T Ziolo Journal: Free Radic Biol Med Date: 2008-04-04 Impact factor: 7.376
Authors: Partha Mukhopadhyay; Mohanraj Rajesh; Béla Horváth; Sándor Bátkai; Ogyi Park; Galin Tanchian; Rachel Y Gao; Vivek Patel; David A Wink; Lucas Liaudet; György Haskó; Raphael Mechoulam; Pál Pacher Journal: Free Radic Biol Med Date: 2011-03-11 Impact factor: 7.376
Authors: Craig B Clark; Yi Zhang; Sean M Martin; L Ray Davies; Linjing Xu; Kevin C Kregel; Francis J Miller; Garry R Buettner; Richard E Kerber Journal: Resuscitation Date: 2004-03 Impact factor: 5.262