| Literature DB >> 9463375 |
W Witke1, A V Podtelejnikov, A Di Nardo, J D Sutherland, C B Gurniak, C Dotti, M Mann.
Abstract
Profilins are thought to be essential for regulation of actin assembly. However, the functions of profilins in mammalian tissues are not well understood. In mice profilin I is expressed ubiquitously while profilin II is expressed at high levels only in brain. In extracts from mouse brain, profilin I and profilin II can form complexes with regulators of endocytosis, synaptic vesicle recycling and actin assembly. Using mass spectrometry and database searching we characterized a number of ligands for profilin I and profilin II from mouse brain extracts including dynamin I, clathrin, synapsin, Rho-associated coiled-coil kinase, the Rac-associated protein NAP1 and a member of the NSF/sec18 family. In vivo, profilins co-localize with dynamin I and synapsin in axonal and dendritic processes. Our findings strongly suggest that in brain profilin I and profilin II complexes link the actin cytoskeleton and endocytic membrane flow, directing actin and clathrin assembly to distinct membrane domains.Entities:
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Year: 1998 PMID： 9463375 PMCID： PMC1170446 DOI： 10.1093/emboj/17.4.967
Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598