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Literature DB >> 9460244

Refined solution structure of the DNA-binding domain of GAL4 and use of 3J(113Cd,1H) in structure determination.

Abstract

We have refined the solution structure of cadmium-bound GAL4 and present its 15N and 1H NMR assignments. The root-mean-square (rms) deviation to the average structure was 0.4 +/- 0.05 A for backbone atoms, and 0.9 +/- 0.1 A for all heavy atoms. The three-bond heteronuclear 3J(113Cd,1H) coupling constants were found to disobey a Karplus-type relationship, which was attributable to the unusual constraints imposed by the bimetal-thiolate cluster in GAL4. We conclude that the structural parameters that correlate to 3J(113Cd,1H) are complex.

Entities: Chemical Gene

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Year: 1997 PMID： 9460244 DOI： 10.1023/a:1018332327565

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

15 in total

1. Internal mobility in the partially folded DNA binding and dimerization domains of GAL4: NMR analysis of the N-H spectral density functions.

Authors: J F Lefevre; K T Dayie; J W Peng; G Wagner
Journal: Biochemistry Date: 1996-02-27 Impact factor: 3.162

2. Solution structure of the DNA-binding domain of Cd2-GAL4 from S. cerevisiae.

Authors: J D Baleja; R Marmorstein; S C Harrison; G Wagner
Journal: Nature Date: 1992-04-02 Impact factor: 49.962

3. Structure of the DNA-binding domain of zinc GAL4.

Authors: P J Kraulis; A R Raine; P L Gadhavi; E D Laue
Journal: Nature Date: 1992-04-02 Impact factor: 49.962

4. Sequential assignments of the 1H NMR resonances of Zn(II)2 and 113Cd(II)2 derivatives of the DNA-binding domain of the GAL4 transcription factor reveal a novel structural motif for specific DNA recognition.

Authors: T Pan; J E Coleman
Journal: Biochemistry Date: 1991-04-30 Impact factor: 3.162

5. Structure of the binuclear metal-binding site in the GAL4 transcription factor.

Authors: K H Gardner; T Pan; S Narula; E Rivera; J E Coleman
Journal: Biochemistry Date: 1991-11-26 Impact factor: 3.162

6. Assignment of 1H, 15N, and 13C resonances, identification of elements of secondary structure and determination of the global fold of the DNA-binding domain of GAL4.

Authors: M Shirakawa; W J Fairbrother; Y Serikawa; T Ohkubo; Y Kyogoku; P E Wright
Journal: Biochemistry Date: 1993-03-09 Impact factor: 3.162

7. 113CD-1H spin-spin couplings in homonuclear 1H correlated spectroscopy of metallothionein. Identification of the cysteine 1H spin systems.

Authors: D Neuhaus; G Wagner; M Vasák; J H Kägi; K Wüthrich
Journal: Eur J Biochem Date: 1984-09-17

8. Hydrogen bonds involving sulfur atoms in proteins.

Authors: L M Gregoret; S D Rader; R J Fletterick; F E Cohen
Journal: Proteins Date: 1991

9. Effects of DNA binding and metal substitution on the dynamics of the GAL4 DNA-binding domain as studied by amide proton exchange.

Authors: T Mau; J D Baleja; G Wagner
Journal: Protein Sci Date: 1992-11 Impact factor: 6.725

Refined solution structure of the DNA-binding domain of GAL4 and use of 3J(113Cd,1H) in structure determination.

1. Internal mobility in the partially folded DNA binding and dimerization domains of GAL4: NMR analysis of the N-H spectral density functions.

2. Solution structure of the DNA-binding domain of Cd2-GAL4 from S. cerevisiae.

3. Structure of the DNA-binding domain of zinc GAL4.

4. Sequential assignments of the 1H NMR resonances of Zn(II)2 and 113Cd(II)2 derivatives of the DNA-binding domain of the GAL4 transcription factor reveal a novel structural motif for specific DNA recognition.

5. Structure of the binuclear metal-binding site in the GAL4 transcription factor.

6. Assignment of 1H, 15N, and 13C resonances, identification of elements of secondary structure and determination of the global fold of the DNA-binding domain of GAL4.

7. 113CD-1H spin-spin couplings in homonuclear 1H correlated spectroscopy of metallothionein. Identification of the cysteine 1H spin systems.

8. Hydrogen bonds involving sulfur atoms in proteins.

9. Effects of DNA binding and metal substitution on the dynamics of the GAL4 DNA-binding domain as studied by amide proton exchange.

10. Sequence-specific 1H-NMR assignments in rabbit-liver metallothionein-2.

Review 1. Structural features specific to plant metallothioneins.

2. Protein and metal cluster structure of the wheat metallothionein domain γ-E(c)-1: the second part of the puzzle.

Review 3. Engineered biological entities for drug delivery and gene therapy protein nanoparticles.

Review 4. A Glimpse to Background and Characteristics of Major Molecular Biological Networks.