Effect of dibutyryl cAMP and several reagents on apoptosis in PC12 cells induced by a sialoglycopeptide from bovine brain.

The sialoglycopeptide (SGP) prepared from bovine brain induces apoptosis in rat pheochromocytoma (PC12) cells. This apoptosis accompanies a decrease in cell growth, DNA fragmentation to oligonucleotide repeats, and morphological changes involving cell shrinkage. Although the growth of PC12 cells was maintained by nerve growth factor (NGF) or basic fibroblast growth factor (bFGF), the apoptosis induced by SGP occurred in the presence of these reagents. The addition of macromolecule synthesis inhibitors or depolarization of membrane potential by extracellular K+ did not prevent apoptosis. Apoptosis was prevented only by a cAMP analog, dibutyryl cAMP, or high concentrations of serum.