K Xiang1, T Zheng, D Sun, J Li. 1. Medical Genetics Research Laboratory, Shanghai Sixth People Hospital, Shanghai, 200233 P. R. China.
Abstract
OBJECTIVE: To ascertain whether the angiotensin II type 1 receptor (AGTR1) gene is associated with coronary heart disease (CHD), hypertension (HTN) and non-insulin-dependent diabetes mellitus (NIDDM) in Chinese and to observe the interaction of AGTR1 gene and angiotensin I converting enzyme(ACE) gene to the development of the associated disease in Chinese. METHODS: 270 cases of Chinese with CHD, HTN or NIDDM in single or in different combinations were genotyped for the A1166C variation at the 3'-untranslated region of AGTR1 gene by using PCR/Dde I restriction endonuclease digestion. And also, the insertion/deletion polymorphism at the intron 16 of ACE gene was typed by PCR. RESULTS: (1) AGTR1 gene was associated with CHD (logistic regression analysis, P=0.02), but not with HTN and NIDDM; (2) The attributable risk of AGTR1 gene for CHD was 12.5% and the odds ratio was 4.55. Both AGTR1 gene and ACE gene were independent variation factors for CHD (P=0.032 and P=0.002 respectively); (3) The contribution of AGTR1 gene to the development of CHD was independent of total body adiposity, blood lipid profile and blood pressure; (4) The analysis of individual combined genotypes of AGTR1 gene and ACE gene showed significant difference in frequency distribution between patients with CHD and controls. CONCLUSION: AGTR1 gene contributes to the development of CHD and is an independent risk factor for CHD in Chinese.
OBJECTIVE: To ascertain whether the angiotensin II type 1 receptor (AGTR1) gene is associated with coronary heart disease (CHD), hypertension (HTN) and non-insulin-dependent diabetes mellitus (NIDDM) in Chinese and to observe the interaction of AGTR1 gene and angiotensin I converting enzyme(ACE) gene to the development of the associated disease in Chinese. METHODS: 270 cases of Chinese with CHD, HTN or NIDDM in single or in different combinations were genotyped for the A1166C variation at the 3'-untranslated region of AGTR1 gene by using PCR/Dde I restriction endonuclease digestion. And also, the insertion/deletion polymorphism at the intron 16 of ACE gene was typed by PCR. RESULTS: (1) AGTR1 gene was associated with CHD (logistic regression analysis, P=0.02), but not with HTN and NIDDM; (2) The attributable risk of AGTR1 gene for CHD was 12.5% and the odds ratio was 4.55. Both AGTR1 gene and ACE gene were independent variation factors for CHD (P=0.032 and P=0.002 respectively); (3) The contribution of AGTR1 gene to the development of CHD was independent of total body adiposity, blood lipid profile and blood pressure; (4) The analysis of individual combined genotypes of AGTR1 gene and ACE gene showed significant difference in frequency distribution between patients with CHD and controls. CONCLUSION:AGTR1 gene contributes to the development of CHD and is an independent risk factor for CHD in Chinese.
Authors: Praveen Sethupathy; Christelle Borel; Maryline Gagnebin; Gregory R Grant; Samuel Deutsch; Terry S Elton; Artemis G Hatzigeorgiou; Stylianos E Antonarakis Journal: Am J Hum Genet Date: 2007-07-12 Impact factor: 11.025
Authors: J J McCarthy; A Parker; R Salem; D J Moliterno; Q Wang; E F Plow; S Rao; G Shen; W J Rogers; L K Newby; R Cannata; K Glatt; E J Topol Journal: J Med Genet Date: 2004-05 Impact factor: 6.318