Literature DB >> 9453659

Anti-lipid A monoclonal antibody centoxin (HA-1A) binds to a wide variety of hydrophobic ligands.

E J Helmerhorst1, J J Maaskant, B J Appelmelk.   

Abstract

This note describes the binding specificities of four lipid A monoclonal antibodies (MAbs) including Centoxin (HA-1A); these MAbs display similar binding properties. MAbs reacted with lipid A and heat-killed smooth bacteria, whereas no reactivity was observed with smooth lipopolysaccharide (LPS). Immunoblotting of bacterial extracts separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the MAbs bound to many polypeptide bands including the molecular weight markers. Denaturation of bovine serum albumin (BSA) by boiling or dithiothreitol treatment unmasked antibody epitopes. In addition, binding both to a hydrophobic aliphatic C12 chain covalently coupled to BSA and to single-stranded DNA was observed. The polyreactivity of these clones is most likely mediated by a preferential reactivity with hydrophobic molecular patches.

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Year:  1998        PMID： 9453659      PMCID： PMC107987     

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  18 in total

1.  Characterization of the epitope specificity of murine monoclonal antibodies directed against lipid A.

Authors:  H M Kuhn; L Brade; B J Appelmelk; S Kusumoto; E T Rietschel; H Brade
Journal:  Infect Immun       Date:  1992-06       Impact factor: 3.441

2.  Antibiotics enhance binding by human lipid A-reactive monoclonal antibody HA-1A to smooth gram-negative bacteria.

Authors:  S A Siegel; M E Evans; M Pollack; A O Leone; C S Kinney; S H Tam; P E Daddona
Journal:  Infect Immun       Date:  1993-02       Impact factor: 3.441

3.  Anti-endotoxin human monoclonal antibody A6H4C5 (HA-1A) utilizes the VH4.21 gene.

Authors:  M M Bieber; N M Bhat; N N Teng
Journal:  Clin Infect Dis       Date:  1995-10       Impact factor: 9.079

4.  A comparative study on the effectiveness of various procedures for attachment of two proteins (L-asparaginase and horse radish peroxidase) to the surface of liposomes.

Authors:  E Claassen; N van Rooijen
Journal:  Prep Biochem       Date:  1983

5.  An enzyme-linked immunosorbent assay (ELISA) for the measurement of antibodies to different parts of the gram-negative lipopolysaccharide core region.

Authors:  B J Appelmelk; A M Verweij-Van Vught; D M MacLaren; L G Thijs
Journal:  J Immunol Methods       Date:  1985-10-10       Impact factor: 2.303

6.  Characterization of specific binding of a human immunoglobulin M monoclonal antibody to lipopolysaccharide and its lipid A domain.

Authors:  Y Fujihara; M G Lei; D C Morrison
Journal:  Infect Immun       Date:  1993-03       Impact factor: 3.441

7.  A controlled trial of HA-1A in a canine model of gram-negative septic shock.

Authors:  Z M Quezado; C Natanson; D W Alling; S M Banks; C A Koev; R J Elin; J M Hosseini; J D Bacher; R L Danner; W D Hoffman
Journal:  JAMA       Date:  1993-05-05       Impact factor: 56.272

8.  Reactivity of the human antiendotoxin immunoglobulin M monoclonal antibody HA-1A with lipopolysaccharides from rough and smooth gram-negative organisms.

Authors:  M A Mascelli; B Frederick; T Ely; D S Neblock; D J Shealy; K Y Pak; P E Daddona
Journal:  Infect Immun       Date:  1993-05       Impact factor: 3.441

9.  Treatment of gram-negative bacteremia and septic shock with HA-1A human monoclonal antibody against endotoxin. A randomized, double-blind, placebo-controlled trial. The HA-1A Sepsis Study Group.

Authors:  E J Ziegler; C J Fisher; C L Sprung; R C Straube; J C Sadoff; G E Foulke; C H Wortel; M P Fink; R P Dellinger; N N Teng
Journal:  N Engl J Med       Date:  1991-02-14       Impact factor: 91.245

10.  Production and characterisation of mouse monoclonal antibodies reacting with the lipopolysaccharide core region of gram-negative bacilli.

Authors:  B J Appelmelk; A M Verweij-van Vught; J J Maaskant; W F Schouten; A J De Jonge; L G Thijs; D M Maclaren
Journal:  J Med Microbiol       Date:  1988-06       Impact factor: 2.472
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  12 in total

Review 1.  Back to the future: antibody-based strategies for the treatment of infectious diseases.

Authors:  H Barbaros Oral; Cüneyt Ozakin; Cezmi A Akdiş
Journal:  Mol Biotechnol       Date:  2002-07       Impact factor: 2.695

2.  Top Down Tandem Mass Spectrometric Analysis of a Chemically Modified Rough-Type Lipopolysaccharide Vaccine Candidate.

Authors:  Benjamin L Oyler; Mohd M Khan; Donald F Smith; Erin M Harberts; David P A Kilgour; Robert K Ernst; Alan S Cross; David R Goodlett
Journal:  J Am Soc Mass Spectrom       Date:  2018-02-20       Impact factor: 3.109

3.  Structural Basis for Antibody Recognition of Lipid A: INSIGHTS TO POLYSPECIFICITY TOWARD SINGLE-STRANDED DNA.

Authors:  Omid Haji-Ghassemi; Sven Müller-Loennies; Teresa Rodriguez; Lore Brade; Paul Kosma; Helmut Brade; Stephen V Evans
Journal:  J Biol Chem       Date:  2015-06-17       Impact factor: 5.157

4.  Role of phosphatidylinositol mannosides in the interaction between mycobacteria and DC-SIGN.

Authors:  Nicole N Driessen; Roy Ummels; Janneke J Maaskant; Sudagar S Gurcha; Gurdyal S Besra; Gary D Ainge; David S Larsen; Gavin F Painter; Christina M J E Vandenbroucke-Grauls; Jeroen Geurtsen; Ben J Appelmelk
Journal:  Infect Immun       Date:  2009-08-03       Impact factor: 3.441

5.  The Combining Sites of Anti-lipid A Antibodies Reveal a Widely Utilized Motif Specific for Negatively Charged Groups.

Authors:  Omid Haji-Ghassemi; Sven Müller-Loennies; Teresa Rodriguez; Lore Brade; Hans-Dieter Grimmecke; Helmut Brade; Stephen V Evans
Journal:  J Biol Chem       Date:  2016-03-01       Impact factor: 5.157

6.  Zwitterionic chitosan for the systemic treatment of sepsis.

Authors:  Eun Jung Cho; Kyung-Oh Doh; Jinho Park; Hyesun Hyun; Erin M Wilson; Paul W Snyder; Michael D Tsifansky; Yoon Yeo
Journal:  Sci Rep       Date:  2016-07-14       Impact factor: 4.379

Review 7.  A Dormant Microbial Component in the Development of Preeclampsia.

Authors:  Douglas B Kell; Louise C Kenny
Journal:  Front Med (Lausanne)       Date:  2016-11-29

8.  The antigenicity and cholesteroid nature of mycolic acids determined by recombinant chicken antibodies.

Authors:  Heena Ranchod; Fortunate Ndlandla; Yolandy Lemmer; Mervyn Beukes; Johann Niebuhr; Juma Al-Dulayymi; Susan Wemmer; Jeanni Fehrsen; Mark Baird; Jan Verschoor
Journal:  PLoS One       Date:  2018-08-09       Impact factor: 3.240

Review 9.  To What Extent Are the Terminal Stages of Sepsis, Septic Shock, Systemic Inflammatory Response Syndrome, and Multiple Organ Dysfunction Syndrome Actually Driven by a Prion/Amyloid Form of Fibrin?

Authors:  Douglas B Kell; Etheresia Pretorius
Journal:  Semin Thromb Hemost       Date:  2017-08-04       Impact factor: 4.180

10.  Low-dose polymyxin: an option for therapy of Gram-negative sepsis.

Authors:  Stephan Harm; Franz Gabor; Jens Hartmann
Journal:  Innate Immun       Date:  2016-03-17       Impact factor: 2.680
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