Literature DB >> 9449553

Improved determination of urinary cortisol and cortisone, or corticosterone and 11-dehydrocorticosterone by high-performance liquid chromatography with ultraviolet absorbance detection.

M Hay1, P Mormède.   

Abstract

A sensitive assay was developed for the determination of low levels of free (unconjugated) glucocorticoids in human, swine (cortisol and cortisone) and rat urine (corticosterone and 11-dehydrocorticosterone), using solid-phase extraction and HPLC with UV absorbance detection (254 nm). Precise quantitation is allowed by the use of internal standards (dexamethasone for swine urine and Reichstein's substance S for rat urine). This simple method allows the use of small urine samples (less than 2 ml), and is suitable for a wide range of applications in human and animal clinical and physiological studies.

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Year:  1997        PMID： 9449553     DOI： 10.1016/s0378-4347(97)00361-7

Source DB:  PubMed          Journal:  J Chromatogr B Biomed Sci Appl        ISSN： 1387-2273


  5 in total

1.  A cross-sectional study evaluating the relationship between cortisol suppression and asthma control in patients with difficult asthma.

Authors:  S Aburuz; L G Heaney; J Millership; J Gamble; J McElnay
Journal:  Br J Clin Pharmacol       Date:  2007-01       Impact factor: 4.335

2.  Measurement of 11-dehydrocorticosterone in mice, rats and songbirds: Effects of age, sex and stress.

Authors:  Jordan E Hamden; Melody Salehzadeh; Cecilia Jalabert; Timothy P O'Leary; Jason S Snyder; Celso E Gomez-Sanchez; Kiran K Soma
Journal:  Gen Comp Endocrinol       Date:  2019-05-27       Impact factor: 2.822

3.  Oral contraception but not menstrual cycle phase is associated with increased free cortisol levels and low hypothalamo-pituitary-adrenal axis reactivity.

Authors:  N Boisseau; C Enea; V Diaz; B Dugué; J B Corcuff; M Duclos
Journal:  J Endocrinol Invest       Date:  2013-05-22       Impact factor: 4.256

4.  A revised role for P-glycoprotein in the brain distribution of dexamethasone, cortisol, and corticosterone in wild-type and ABCB1A/B-deficient mice.

Authors:  Brittany L Mason; Carmine M Pariante; Sarah A Thomas
Journal:  Endocrinology       Date:  2008-06-12       Impact factor: 4.736

5.  Multifactorial monitoring of training load in elite rugby sevens players: cortisol/cortisone ratio as a valid tool of training load monitoring.

Authors:  T Bouaziz; E Makni; P Passelergue; Z Tabka; G Lac; W Moalla; K Chamari; M Elloumi
Journal:  Biol Sport       Date:  2016-05-10       Impact factor: 2.806
  5 in total

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