Literature DB >> 944919

Isolation of single stranded DNA related to the transcriptional activity of animal cells.

H Tapiero, S A Leibowitch, D Shaool, M N Monier, J Harel.   

Abstract

Single stranded DNA (s.s.DNA) comprising 1-2% of the total nuclear DNA was isolated by an improved method of hydroxyapatite chromatography from native nuclear DNA3 of embryonic chick cells, labeled for several cell generations with 3H-thymidine. Small quantities of 3H-DNA were annealed with a large excess of unlabeled DNA or polysomal RNA from chick embryos. Hybridization kinetics (monitored by the use of SI nuclease digestion, hydroxyapatite chromatography and thermalfusion), indicated that s.s.DNA belongs to the non repetitious fraction of the cell genome. One third represents DNA sequences engaged in the transcription of messenger RNA's.

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Year:  1976        PMID： 944919      PMCID： PMC342957          DOI： 10.1093/nar/3.4.953

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

1.  SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.

Authors:  F W STUDIER
Journal:  J Mol Biol       Date:  1965-02       Impact factor: 5.469

2.  On the structure of the folded chromosome of Escherichia coli.

Authors:  A Worcel; E Burgi
Journal:  J Mol Biol       Date:  1972-11-14       Impact factor: 5.469

3.  Fractions of Chinese hamster DNA differing in their content of guanine+cytosine and evidence for the presence of single-stranded DNA.

Authors:  H Tapiero; R Caneva; C L Schildkraut
Journal:  Biochim Biophys Acta       Date:  1972-07-20

4.  Regulation of the nucleolar DNA-dependent RNA polymerase by amino acids in Ehrlich ascites tumor cells.

Authors:  M T Franze-Fernández; A O Pogo
Journal:  Proc Natl Acad Sci U S A       Date:  1971-12       Impact factor: 11.205

5.  Replication of repetitious DNA in synchronized chick fibroblast cells.

Authors:  H Tapiero; D Shaool; M N Monier; J Harel
Journal:  Exp Cell Res       Date:  1974-11       Impact factor: 3.905

6.  Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.

Authors:  R J Britten; D E Kohne
Journal:  Science       Date:  1968-08-09       Impact factor: 47.728

7.  Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains.

Authors:  R Okazaki; T Okazaki; K Sakabe; K Sugimoto; A Sugino
Journal:  Proc Natl Acad Sci U S A       Date:  1968-02       Impact factor: 11.205

8.  Breaks in DNA stimulate transcription by core RNA polymerase.

Authors:  V Vogt
Journal:  Nature       Date:  1969-08-23       Impact factor: 49.962

9.  Distribution of repetitious sequences in chick nuclear DNA.

Authors:  H Tapiero; M N Monier; D Shaool; J Harel
Journal:  Nucleic Acids Res       Date:  1974-02       Impact factor: 16.971

10.  General model for the chromosomes of higher organisms.

Authors:  F Crick
Journal:  Nature       Date:  1971-11-05       Impact factor: 49.962
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  4 in total

1.  Active DNA transcription sites released from the genome of normal embryonic chicken cells.

Authors:  S A Leibovitch; J Harel
Journal:  Nucleic Acids Res       Date:  1978-03       Impact factor: 16.971

2.  Quantitative amplification of single-stranded DNA (QAOS) demonstrates that cdc13-1 mutants generate ssDNA in a telomere to centromere direction.

Authors:  C Booth; E Griffith; G Brady; D Lydall
Journal:  Nucleic Acids Res       Date:  2001-11-01       Impact factor: 16.971

3.  Structural defects in rat liver deoxyribonucleic acid. Endogenous single-strained regions in comparison with damage induced in vivo by a carcinogen.

Authors:  B W Stewart; P H Huang; M J Brian
Journal:  Biochem J       Date:  1979-05-01       Impact factor: 3.857

4.  Single-stranded DNA from oncornavirus-infected cells enriched in virus-specific DNA sequences.

Authors:  S A Leibovitch; H Tapiero; J Harel
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205
  4 in total

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