OBJECTIVE: To study the specificity of antibodies reactive with renal antigens in mixed cryoglobulinemia. METHODS: Sera from mixed cryoglobulinemia (MC) patients were tested on human kidney extracts by immunoblot. A partially purified renal antigen was subjected to N-terminal sequencing. RESULTS: Antibodies reactive with a renal antigen of 48 kD were detected in 7 out of 11 patients with MC and renal involvement. N-terminal sequencing of this antigen showed that it was identical with alpha-enolase. This result was confirmed by the reactivity of the renal antigen with a rabbit anti-serum specific for alpha-enolase. CONCLUSIONS: The results indicate that antibodies specific for alpha-enolase are frequently produced by mixed cryoglobulinemia patients with renal involvement.
OBJECTIVE: To study the specificity of antibodies reactive with renal antigens in mixed cryoglobulinemia. METHODS: Sera from mixed cryoglobulinemia (MC) patients were tested on human kidney extracts by immunoblot. A partially purified renal antigen was subjected to N-terminal sequencing. RESULTS: Antibodies reactive with a renal antigen of 48 kD were detected in 7 out of 11 patients with MC and renal involvement. N-terminal sequencing of this antigen showed that it was identical with alpha-enolase. This result was confirmed by the reactivity of the renal antigen with a rabbit anti-serum specific for alpha-enolase. CONCLUSIONS: The results indicate that antibodies specific for alpha-enolase are frequently produced by mixed cryoglobulinemiapatients with renal involvement.
Authors: David S Ucker; Mohit Raja Jain; Goutham Pattabiraman; Karol Palasiewicz; Raymond B Birge; Hong Li Journal: J Biol Chem Date: 2012-01-18 Impact factor: 5.157
Authors: L Musante; G Candiano; M Bruschi; L Santucci; B Carnemolla; P Orecchia; M Giampuzzi; C Zennaro; S Sanna-Cherchi; M Carraro; R Oleggini; G Camussi; F Perfumo; G M Ghiggeri Journal: Clin Exp Immunol Date: 2005-09 Impact factor: 4.330
Authors: Maurizio Bruschi; Renato Alberto Sinico; Gabriella Moroni; Federico Pratesi; Paola Migliorini; Maricla Galetti; Corrado Murtas; Angela Tincani; Michael Madaio; Antonella Radice; Franco Franceschini; Barbara Trezzi; Laura Bianchi; Agata Giallongo; Rita Gatti; Regina Tardanico; Andrea Scaloni; Chiara D'Ambrosio; Maria Luisa Carnevali; Piergiorgio Messa; Pietro Ravani; Giancarlo Barbano; Beatrice Bianco; Alice Bonanni; Francesco Scolari; Alberto Martini; Giovanni Candiano; Landino Allegri; Gian Marco Ghiggeri Journal: J Am Soc Nephrol Date: 2014-05-01 Impact factor: 10.121