Literature DB >> 9441298

[AccBSI - a novel restriction endonuclease from Acinetobacter calcoaceticus BS].

M A Abdurashitov, E V Kiseleva, T V Miakisheva, V S Dedkov, A V Shevchenko, C Kh Degtiarev.   

Abstract

The recognition site of a new restriction endonuclease from Acinetobacter calcoaceticus BS was determined. This is a nonpalindromic sequence. AccBSI restrictase cleaves DNA chains in the middle of the recognition sequence; therefore, ligation of its digestion fragments restored AccBSI recognition sites and generated palindromic sequences recognized by SacI and SacII restrictases.

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Year:  1997        PMID: 9441298

Source DB:  PubMed          Journal:  Prikl Biokhim Mikrobiol        ISSN: 0555-1099


  1 in total

1.  BtrI, a novel restriction endonuclease, recognises the non-palindromic sequence 5'-CACGTC(-3/-3)-3'.

Authors:  S K Degtyarev; O A Belichenko; N A Lebedeva; V S Dedkov; M A Abdurashitov
Journal:  Nucleic Acids Res       Date:  2000-06-01       Impact factor: 16.971

  1 in total

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