Literature DB >> 9438255

A micro-scale method for determining relative metal-binding affinities of proteins.

J Sommer-Knudsen1, A Bacic.   

Abstract

This article describes a quick and easy method for determining relative binding affinities between proteins and metal ions. The method is based on separating unbound metal ions from metal ions bound to protein by ultrafiltration using microcentrifuge ultrafiltration units. Bovine serum albumin (BSA) was used as the test protein and the relative affinity towards divalent metal ions was found to be Cu2+ > Zn2+ > Cd2+ > Pb2+ > Ni2+ > Co2+, which corresponds to the relative orders reported in the literature.

Entities:  

Mesh：

Substances：

Year:  1997        PMID： 9438255     DOI： 10.1007/BF02760774

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  6 in total

1.  Thermodynamic proton-, cadmium-, and zinc-binding constants of serum albumin determined by zone electrophoresis.

Authors:  H WALDMANN-MEYER
Journal:  J Biol Chem       Date:  1960-11       Impact factor: 5.157

2.  Elimination of adventitious metals.

Authors:  B Holmquist
Journal:  Methods Enzymol       Date:  1988       Impact factor: 1.600

3.  The initial equilibrium steps in the interactions of bovine plasma albumin and zinc(II) ions. A potentiostatic study.

Authors:  R Osterberg
Journal:  Acta Chem Scand       Date:  1971

4.  Binding of calcium to serum albumin. I. Stoichiometry and intrinsic association constant at physiological pH, ionic strength, and temperature.

Authors:  K O Pedersen
Journal:  Scand J Clin Lab Invest       Date:  1971-12       Impact factor: 1.713

5.  Intrinsic stoichiometric equilibrium constants for the binding of zinc(II) and copper(II) to the high affinity site of serum albumin.

Authors:  J Masuoka; J Hegenauer; B R Van Dyke; P Saltman
Journal:  J Biol Chem       Date:  1993-10-15       Impact factor: 5.157

6.  Copper and zinc ion binding by bovine, dog, and rat serum albumins.

Authors:  E Giroux; J Schoun
Journal:  J Inorg Biochem       Date:  1981-07       Impact factor: 4.155
  6 in total
  1 in total

1.  Regulation of connexin36 gap junction channels by n-alkanols and arachidonic acid.

Authors:  Alina Marandykina; Nicolás Palacios-Prado; Lina Rimkutė; Vytenis A Skeberdis; Feliksas F Bukauskas
Journal:  J Physiol       Date:  2013-02-18       Impact factor: 5.182
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.