SETTING: An effective oral bacille Calmette-Guérin (BCG) vaccine would have advantages for use in humans and as an oral bait vaccine for protecting wild-life against bovine tuberculosis. OBJECTIVE: To compare the level of protection against tuberculosis in intraduodenally BCG-vaccinated possums with those vaccinated intragastrically in order to determine whether degradation of BCG in the stomach lowers vaccine efficacy. DESIGN: Three groups of five possums were vaccinated with BCG by the intraduodenal, intragastric or subcutaneous routes, with a fourth group serving as unvaccinated controls. The animals were later challenged intratracheally with a low dose of virulent Mycobacterium bovis. RESULTS: Possums vaccinated intraduodenally with BCG had significantly greater lymphocyte blastogenic responses to bovine purified protein derivative (PPD) and lower lung bacterial counts in comparison with intragastrically vaccinated animals. In comparison with unvaccinated animals, all of the BCG-vaccinated groups had significant protection against M. bovis infection as assessed by changes in body weight, lung weight and reduction in numbers of mycobacteria and granulomas in the spleen. CONCLUSION: The enhanced immune responses and protection against bovine tuberculosis observed in the intraduodenally BCG-vaccinated possums indicated that if BCG vaccine is protected from degradation in the stomach its efficacy should improve.
SETTING: An effective oral bacille Calmette-Guérin (BCG) vaccine would have advantages for use in humans and as an oral bait vaccine for protecting wild-life against bovinetuberculosis. OBJECTIVE: To compare the level of protection against tuberculosis in intraduodenally BCG-vaccinated possums with those vaccinated intragastrically in order to determine whether degradation of BCG in the stomach lowers vaccine efficacy. DESIGN: Three groups of five possums were vaccinated with BCG by the intraduodenal, intragastric or subcutaneous routes, with a fourth group serving as unvaccinated controls. The animals were later challenged intratracheally with a low dose of virulent Mycobacterium bovis. RESULTS: Possums vaccinated intraduodenally with BCG had significantly greater lymphocyte blastogenic responses to bovine purified protein derivative (PPD) and lower lung bacterial counts in comparison with intragastrically vaccinated animals. In comparison with unvaccinated animals, all of the BCG-vaccinated groups had significant protection against M. bovis infection as assessed by changes in body weight, lung weight and reduction in numbers of mycobacteria and granulomas in the spleen. CONCLUSION: The enhanced immune responses and protection against bovinetuberculosis observed in the intraduodenally BCG-vaccinated possums indicated that if BCG vaccine is protected from degradation in the stomach its efficacy should improve.
Authors: F E Aldwell; B L Dicker; F M da Silva Tatley; M F Cross; S Liggett; C G Mackintosh; J F Griffin Journal: Infect Immun Date: 2000-12 Impact factor: 3.441
Authors: Simon Clark; Martin L Cross; Allan Nadian; Julia Vipond; Pinar Court; Ann Williams; R Glyn Hewinson; Frank E Aldwell; Mark A Chambers Journal: Infect Immun Date: 2008-06-02 Impact factor: 3.441
Authors: Gareth A Williams; Marjorie E Koenen; Robert Havenaar; Paul Wheeler; Sonya Gowtage; Sandrine Lesellier; Mark A Chambers Journal: PLoS One Date: 2019-04-19 Impact factor: 3.240
Authors: Sandrine Lesellier; Maria-Laura Boschiroli; Jacques Barrat; Christoph Wanke; Francisco J Salguero; Waldo L Garcia-Jimenez; Alex Nunez; Ana Godinho; John Spiropoulos; Simonette Palmer; Dipesh Dave; Paul Anderson; Jean-Marc Boucher; Krystel de Cruz; Sylvie Henault; Lorraine Michelet; Sonya Gowtage; Gareth A Williams; Allan K Nadian; Elodie Monchâtre-Leroy; Frank Boué; Mark A Chambers; Céline Richomme Journal: BMC Vet Res Date: 2019-12-06 Impact factor: 2.741