S Nakielny1, G Dreyfuss. 1. Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6148, USA.
Abstract
BACKGROUND: Nuclear protein import and export are mediated by receptor proteins that recognize nuclear localization sequences (NLSs) or nuclear export sequences (NESs) and target the NLS-bearing or NES-bearing protein to the nuclear pore complex (NPC). Temperature-dependent translocation of the receptor-cargo complex in both directions through the NPC requires the GTPase Ran, and it has been proposed that the Ran GTPase cycle mediates translocation. We have addressed the role of GTP hydrolysis in these processes by studying the import receptor transportin, which mediates the import of a group of abundant heterogeneous nuclear RNA-binding proteins bearing the M9 NLS. RESULTS: We investigated the transport properties of transportin and found that the carboxy-terminal region of transportin could, by itself, be imported into the nucleus. Transportin import and export were inhibited by low temperature in vitro, but were unaffected by the non-hydrolyzable GTP analogue GMP-PNP. CONCLUSIONS: Temperature-dependent import and export through the NPC can be uncoupled from the Ran GTPase cycle and can occur without GTP hydrolysis.
BACKGROUND: Nuclear protein import and export are mediated by receptor proteins that recognize nuclear localization sequences (NLSs) or nuclear export sequences (NESs) and target the NLS-bearing or NES-bearing protein to the nuclear pore complex (NPC). Temperature-dependent translocation of the receptor-cargo complex in both directions through the NPC requires the GTPase Ran, and it has been proposed that the Ran GTPase cycle mediates translocation. We have addressed the role of GTP hydrolysis in these processes by studying the import receptor transportin, which mediates the import of a group of abundant heterogeneous nuclear RNA-binding proteins bearing the M9 NLS. RESULTS: We investigated the transport properties of transportin and found that the carboxy-terminal region of transportin could, by itself, be imported into the nucleus. Transportin import and export were inhibited by low temperature in vitro, but were unaffected by the non-hydrolyzable GTP analogue GMP-PNP. CONCLUSIONS: Temperature-dependent import and export through the NPC can be uncoupled from the Ran GTPase cycle and can occur without GTP hydrolysis.
Authors: C Kruse; D K Willkomm; A Grünweller; T Vollbrandt; S Sommer; S Busch; T Pfeiffer; J Brinkmann; R K Hartmann; P K Müller Journal: Biochem J Date: 2000-02-15 Impact factor: 3.857