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Literature DB >> 94263

Biochemical and immunological studies of three genetic variants of 3-phosphoglycerate kinase 2 from the mouse.

Abstract

Three electrophoretic variants of 3-phosphoglycerate kinase 2 (PGK-2A,PGK-2B, and PGK-2C) were purified from DBA/2J, C3H/HeJ, and C57L/J mice, respectively. PGK-2C exhibits only 2% of the specific activity of PGK-2A and PGK-2B in the reaction leading to the formation of 1,3-diphosphoglycerate. Compared to PGK-2A and PGK-2B, PGK-2C exhibits broader coenzyme specificity and lower Kms for substrate and coenzymes. Incubation at 45C revealed immunionactivation and double immunodiffusion studies showed that mice carrying any one of these three PGK-2 alleles have similar amounts of proteins for PGK-1 and PGK-2 in testes. The results of these studies suggest that low PGK-2C activity in C57L/J mice is a result of a structural rather than a regulatory gene mutation.

Entities: Chemical Gene Species

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Year: 1979 PMID： 94263 DOI： 10.1007/bf00502123

Source DB: PubMed Journal: Biochem Genet ISSN： 0006-2928 Impact factor: 1.890

16 in total

1. An allelic isozyme of mouse PGK-2 with low activity.

Authors: J L Vandeberg; S V Blohm
Journal: J Exp Zool Date: 1977-09

2. Glucose-6-phosphate dehydrogenase activity changes during spermatogenesis: possible relevance to X-chromosome inactivation.

Authors: R P Erickson
Journal: Dev Biol Date: 1976-10-01 Impact factor: 3.582

7. Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins.

Authors: O Vesterberg; H Svensson
Journal: Acta Chem Scand Date: 1966

8. Kinetic studies on the reaction catalyzed by phosphoglycerate kinase. II. The kinetic relationships between 3-phosphoglycerate, MgATP2-and activating metal ion.

Authors: M Larsson-Raźnikiewicz
Journal: Biochim Biophys Acta Date: 1967-01-11

4. Immunological and biochemical analysis of a null mutant of alpha-glycerolphosphate dehydrogenase from Drosophila melanogaster.

Authors: C Y Lee; S Read; L Johnson
Journal: Mol Cell Biochem Date: 1980-04-18 Impact factor: 3.396

5. Immunohistochemical localization of mouse testis-specific phosphoglycerate kinase (PGK-2) by monoclonal antibodies.

Authors: H Blüthmann; L Cicurel; G W Kuntz; G Haedenkamp; K Illmensee
Journal: EMBO J Date: 1982 Impact factor: 11.598

5 in total

Biochemical and immunological studies of three genetic variants of 3-phosphoglycerate kinase 2 from the mouse.

1. An allelic isozyme of mouse PGK-2 with low activity.

2. Glucose-6-phosphate dehydrogenase activity changes during spermatogenesis: possible relevance to X-chromosome inactivation.

3. Purification and characterization of two isozymes of 3-phosphoglycerate kinase from the mouse.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. 3-phosphoglycerate kinase from rabbit sceletal muscle and yeast.

6. Electrophoretic variation for x-chromosome-linked phosphoglycerate kinase (pgk-1) in the mouse.

7. Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins.

8. Kinetic studies on the reaction catalyzed by phosphoglycerate kinase. II. The kinetic relationships between 3-phosphoglycerate, MgATP2-and activating metal ion.

9. Autosomal phosphoglycerate kinase linked to mouse major histocompatibility complex.

10. Activity and androgenic control of glycolytic enzymes in the epididymis and epididymal spermatozoa of the rat.

1. Wild-derived alleles of five allozyme-encoding loci in B10.W mice.

2. Characterization of two electrophoretic lactate dehydrogenase-A mutants in Mus musculus.

3. Biochemical characterization of phosphoglucose isomerase and genetic variants from mouse and Drosophila melanogaster.

4. Immunological and biochemical analysis of a null mutant of alpha-glycerolphosphate dehydrogenase from Drosophila melanogaster.

5. Immunohistochemical localization of mouse testis-specific phosphoglycerate kinase (PGK-2) by monoclonal antibodies.