Literature DB >> 942370

Metal-replacement studies in Bacillus stearothermophilus aldolase and a comparison of the mechanisms of class I and class II aldolases.

H A Hill, R R Lobb, S L Sharp, A M Stokes, J I Harris, R S Jack.   

Abstract

A comparison of the product-inhibition patterns during cleavage of D-fructose 1,6-diphosphate by aldolases from yeast, rabbit muscle and Bacillus stearothermophilus shows an ordered reaction sequence for all three enzymes, with dihydroxyacetone phosphate the last-leaving product. Addition of Zn2+, Co2+, Fe2+, Mn2+ or Cd2+ ions to the inactive apo-(Bacillus stearothermophilus aldolase) restores activity to different extents, whereas Ni2+, Mg2+ or Cu2+ ions have no effect. The cleavage activity of this aldolase is not enhanced by added K+ ion. The effects of metal replacement on thermal stability, Km and Vmax. are given and the possible role of the metal is discussed in the light of these results.

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Year:  1976        PMID： 942370      PMCID： PMC1172621          DOI： 10.1042/bj1530551

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  24 in total

1.  THE MECHANISM OF ACTION OF ALDOLASES. IV. LYSINE AS THE SUBSTRATE-BINDING SITE.

Authors:  B L HORECKER; P T ROWLEY; E GRAZI; T CHENG; O TCHOLA
Journal:  Biochem Z       Date:  1963

2.  EVOLUTION OF ALDOLASE.

Authors:  W J RUTTER
Journal:  Fed Proc       Date:  1964 Nov-Dec

3.  MECHANISM OF THE ALDOLASE REACTION.

Authors:  I A ROSE; E L O'CONNELL; A H MEHLER
Journal:  J Biol Chem       Date:  1965-04       Impact factor: 5.157

4.  DISTINCTIVE PROPERTIES OF NATIVE AND CARBOXYPEPTIDASE-TREATED ALDOLASES OF RABBIT MUSCLE AND LIVER.

Authors:  P D SPOLTER; R C ADELMAN; S WEINHOUSE
Journal:  J Biol Chem       Date:  1965-03       Impact factor: 5.157

5.  Interaction between rabbit muscle aldolase and dihydroxyacetone phosphate.

Authors:  A H MEHLER; B BLOOM
Journal:  J Biol Chem       Date:  1963-01       Impact factor: 5.157

6.  Studies on the mechanism on the aldolase reaction; isotope exchange reactions of muscle and yeast aldolase.

Authors:  I A ROSE; S V RIEDER
Journal:  J Biol Chem       Date:  1958-03       Impact factor: 5.157

7.  Contamination in trace element analysis and its control.

Authors:  R E THIERS
Journal:  Methods Biochem Anal       Date:  1957

8.  Comparative properties of yeast and muscle aldolase.

Authors:  O C RICHARDS; W J RUTTER
Journal:  J Biol Chem       Date:  1961-12       Impact factor: 5.157

9.  Magnetic resonance studies of the role of the divalent cation in the mechanism of yeast aldolase.

Authors:  A S Mildvan; R D Kobes; W J Rutter
Journal:  Biochemistry       Date:  1971-03-30       Impact factor: 3.162

10.  Catalysis of an oxygen-exchange reaction of fructose 1,6-diphosphate and fructose 1-phosphate with water by rabbit muscle aldolase.

Authors:  P Model; L Ponticorvo; D Rittenberg
Journal:  Biochemistry       Date:  1968-04       Impact factor: 3.162
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  4 in total

1.  Identification of arginine 331 as an important active site residue in the class II fructose-1,6-bisphosphate aldolase of Escherichia coli.

Authors:  S Qamar; K Marsh; A Berry
Journal:  Protein Sci       Date:  1996-01       Impact factor: 6.725

2.  The proton exchange of the pro-S hydrogen atom at C-1 in dihydroxyacetone phosphate and D-fructose 1,6-bisphosphate catalysed by class-I and class-II aldolases.

Authors:  A Galdes; H A Hill
Journal:  Biochem J       Date:  1978-06-01       Impact factor: 3.857

3.  Purification and characterization of the class-II D-fructose 1,6-bisphosphate aldolase from Escherichia coli (Crookes' strain).

Authors:  S A Baldwin; R N Perham; D Stribling
Journal:  Biochem J       Date:  1978-03-01       Impact factor: 3.857

4.  13C nuclear magnetic resonance studies of anaerobic glycolysis in suspensions of yeast cells.

Authors:  J A den Hollander; T R Brown; K Ugurbil; R G Shulman
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205
  4 in total

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