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Literature DB >> 9419370

Discovery of three genes specifically expressed in human prostate by expressed sequence tag database analysis.

G Vasmatzis¹, M Essand, U Brinkmann, B Lee, I Pastan.

Abstract

A procedure is described to discover genes that are specifically expressed in human prostate. The procedure involves searching the expressed sequence tag (EST) database for genes that have many related EST sequences from human prostate cDNA libraries but none or few from nonprostate human libraries. The selected candidate EST clones were tested by RNA dot blots to examine tissue specificity and by Northern blots to examine the transcript size of the corresponding mRNA. The computer analysis identified 15 promising genes that were previously unidentified. When seven of these were examined in an RNA hybridization experiment, three were found to be prostate specific. The genes identified could be useful in the targeted therapy of prostate cancer. The procedure can easily be applied to discover genes specifically expressed in other organs or tumors.

Entities: Disease Species

Mesh：

Substances：
DNA, Complementary

Year: 1998 PMID： 9419370 PMCID： PMC18207 DOI： 10.1073/pnas.95.1.300

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

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Authors: M R Emmert-Buck; R L Strausberg; D B Krizman; M F Bonaldo; R F Bonner; D G Bostwick; M R Brown; K H Buetow; R F Chuaqui; K A Cole; P H Duray; C R Englert; J W Gillespie; S Greenhut; L Grouse; L W Hillier; K S Katz; R D Klausner; V Kuznetzov; A E Lash; G Lennon; W M Linehan; L A Liotta; M A Marra; P J Munson; D K Ornstein; V V Prabhu; C Prang; G D Schuler; M B Soares; C M Tolstoshev; C D Vocke; R H Waterston
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Authors: M R Emmert-Buck; R L Strausberg; D B Krizman; M F Bonaldo; R F Bonner; D G Bostwick; M R Brown; K H Buetow; R F Chuaqui; K A Cole; P H Duray; C R Englert; J W Gillespie; S Greenhut; L Grouse; L W Hillier; K S Katz; R D Klausner; V Kuznetzov; A E Lash; G Lennon; W M Linehan; L A Liotta; M A Marra; P J Munson; D K Ornstein; V V Prabhu; C Prange; G D Schuler; M B Soares; C M Tolstoshev; C D Vocke; R H Waterston
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Journal: Proc Natl Acad Sci U S A Date: 2000-03-28 Impact factor: 11.205

Discovery of three genes specifically expressed in human prostate by expressed sequence tag database analysis.

1. Basic local alignment search tool.

2. Complementary DNA sequencing: expressed sequence tags and human genome project.

3. Generation and analysis of 280,000 human expressed sequence tags.

4. A catalog of cancer genes at the click of a mouse.

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Review 2. Molecular profiling of clinical tissue specimens: feasibility and applications.

3. Shotgun sequencing of the human transcriptome with ORF expressed sequence tags.

4. Prediction of gene function by genome-scale expression analysis: prostate cancer-associated genes.

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