BACKGROUND: Angiogenesis is essential for tumor growth and metastasis. Vascular endothelial growth factor (VEGF) is the most potent angiogenic factor identified to date. TGF beta-1 acts as an indirect angiogenic agent. METHODS: VEGF and TGF beta-1 were measured in the serum of breast cancer patients and age-matched controls and in tumor tissue of cancer patients by ELISA. VEGF protein and mRNA expression by breast tumor cell lines were examined, and the effect of TGF beta-1 on VEGF production in these cells was assessed. RESULTS: VEGF levels were significantly higher (P = .03) in the serum of patients with breast cancer compared to age-matched controls. A positive correlation was found between serum (r = 0.539) and tumor tissue (r = 0.688) levels of VEGF and TGF beta-1. Metastatic MDA-MB-231 breast cancer cells produce more VEGF than do the primary BT474 cells. TGF beta-1 significantly (P < .05) increased production of VEGF. CONCLUSIONS: Breast cancer cells constitutively produce VEGF protein and mRNA. There is a relationship between VEGF and TGF beta-1 levels in breast cancer patients, and TGF beta-1 regulates VEGF expression by breast cancer cells.
BACKGROUND: Angiogenesis is essential for tumor growth and metastasis. Vascular endothelial growth factor (VEGF) is the most potent angiogenic factor identified to date. TGF beta-1 acts as an indirect angiogenic agent. METHODS:VEGF and TGF beta-1 were measured in the serum of breast cancerpatients and age-matched controls and in tumor tissue of cancerpatients by ELISA. VEGF protein and mRNA expression by breast tumor cell lines were examined, and the effect of TGF beta-1 on VEGF production in these cells was assessed. RESULTS:VEGF levels were significantly higher (P = .03) in the serum of patients with breast cancer compared to age-matched controls. A positive correlation was found between serum (r = 0.539) and tumor tissue (r = 0.688) levels of VEGF and TGF beta-1. Metastatic MDA-MB-231 breast cancer cells produce more VEGF than do the primary BT474 cells. TGF beta-1 significantly (P < .05) increased production of VEGF. CONCLUSIONS:Breast cancer cells constitutively produce VEGF protein and mRNA. There is a relationship between VEGF and TGF beta-1 levels in breast cancerpatients, and TGF beta-1 regulates VEGF expression by breast cancer cells.
Authors: Catalina Suarez-Cuervo; Melinda A Merrell; Latania Watson; Kevin W Harris; Eben L Rosenthal; H Kalervo Väänänen; Katri S Selander Journal: Clin Exp Metastasis Date: 2004 Impact factor: 5.150
Authors: Katherine W Reeves; Roberta B Ness; Roslyn A Stone; Joel L Weissfeld; Victor G Vogel; Robert W Powers; Francesmary Modugno; Jane A Cauley Journal: Cancer Causes Control Date: 2008-11-06 Impact factor: 2.506
Authors: Jason Kindrachuk; Victoria Wahl-Jensen; David Safronetz; Brett Trost; Thomas Hoenen; Ryan Arsenault; Friederike Feldmann; Dawn Traynor; Elena Postnikova; Anthony Kusalik; Scott Napper; Joseph E Blaney; Heinz Feldmann; Peter B Jahrling Journal: J Virol Date: 2014-06-18 Impact factor: 5.103