BACKGROUND: The method for mouse vascularized heart transplantation have been described using suture and cuff techniques. Technical problems have limited its widespread use. Here, we describe our method of modified cervical heterotopic transplantation with the cuff technique. METHODS: By using a smaller Teflon cuff (external diameter 0.6 mm, internal diameter 0.4 mm) and superfine-tip forceps, it became possible to directly pull the edge of the carotid artery and evert the proximal end of the artery over the cuff. Similarly, the external jugular vein could be easily everted over a 22-gauge cuff with this direct pulling method. RESULTS: By these modifications, the operation time was reduced. It usually takes 20 min for the donor harvest, 15 min for preparation of the cervical vessels, and 15 min for anastomosis. All procedures from the donor harvest through skin closure of the recipient mice can be completed within 1 hr, and ischemic time is within 25-40 min. CONCLUSIONS: This method can be used to investigate cyclophosphamide-induced tolerance and mechanisms of reperfusion injury.
BACKGROUND: The method for mouse vascularized heart transplantation have been described using suture and cuff techniques. Technical problems have limited its widespread use. Here, we describe our method of modified cervical heterotopic transplantation with the cuff technique. METHODS: By using a smaller Teflon cuff (external diameter 0.6 mm, internal diameter 0.4 mm) and superfine-tip forceps, it became possible to directly pull the edge of the carotid artery and evert the proximal end of the artery over the cuff. Similarly, the external jugular vein could be easily everted over a 22-gauge cuff with this direct pulling method. RESULTS: By these modifications, the operation time was reduced. It usually takes 20 min for the donor harvest, 15 min for preparation of the cervical vessels, and 15 min for anastomosis. All procedures from the donor harvest through skin closure of the recipient mice can be completed within 1 hr, and ischemic time is within 25-40 min. CONCLUSIONS: This method can be used to investigate cyclophosphamide-induced tolerance and mechanisms of reperfusion injury.
Authors: Byoungchol Oh; Georg J Furtmüller; Michael Sosin; Madeline L Fryer; Lawrence J Gottlieb; Michael R Christy; Gerald Brandacher; Amir H Dorafshar Journal: J Vis Exp Date: 2016-01-23 Impact factor: 1.355
Authors: W Li; A C Bribriesco; R G Nava; A A Brescia; A Ibricevic; J H Spahn; S L Brody; J H Ritter; A E Gelman; A S Krupnick; M J Miller; D Kreisel Journal: Mucosal Immunol Date: 2012-05-02 Impact factor: 7.313
Authors: Rupert Oberhuber; Benno Cardini; Markus Kofler; Paul Ritschl; Robert Oellinger; Felix Aigner; Robert Sucher; Stefan Schneeberger; Johann Pratschke; Gerald Brandacher; Manuel Maglione Journal: J Vis Exp Date: 2014-10-12 Impact factor: 1.355
Authors: Rupert Oberhuber; Gregor Riede; Benno Cardini; David Bernhard; Barbara Messner; Katrin Watschinger; Christina Steger; Gerald Brandacher; Johann Pratschke; Georg Golderer; Ernst R Werner; Manuel Maglione Journal: Sci Rep Date: 2016-11-24 Impact factor: 4.379