| Literature DB >> 9413522 |
C P Czerny1, C Zeller-Lue, A M Eis-Hübinger, O R Kaaden, H Meyer.
Abstract
In August 1990 an orthopox virus (OPV) had been isolated from a severe case of a generalized infection with lethal outcome in an immunosuppressed 18-year-old man. In this communication we present a detailed characterization of the causative virus strain. Based on distinct epitope configurations detected by various monoclonal antibodies the isolate could be differentiated from other OPV species and was classified as a cowpox virus (CP). This classification was confirmed by a species-specific PCR assay and by establishing physical maps for the restriction enzymes HindIII and XhoI. Based on serological data of neutralization assays, blocking-ELISAs and Western blotting analysis evidence is provided that the infection had been acquired from a stray cat.Entities:
Mesh：
Substances：
Year: 1997 PMID： 9413522 DOI： 10.1007/978-3-7091-6534-8_2
Source DB: PubMed Journal: Arch Virol Suppl ISSN： 0939-1983