BACKGROUND: The vascular endothelium plays a critical role in cancer metastasis by directing circulating cancer cells into extravascular tissues and by expressing cell adhesion molecules. The authors investigated the interaction between a cell line derived from human colon cancer and cultured murine endothelial cells, in vitro, and in vivo. MATERIALS AND METHODS: Variant cell lines with high (WiDr-HA) or low (WiDr-LA) levels of cell surface sialyl Lewis(a) antigen (s-Le(a)) were isolated from the heterogeneous WiDr cells (WiDr-P) in order to characterize the biological behavior of colon cancer cells having elevated cell surface contents of s-Le(a). RESULTS: A correlation was found to exist between the degree of s-Le(a) expression, and the attachment of cancer cells to activated human umbilical vein endothelial cells, or to F-2 cells isolated from murine vascular endothelial cells. The adhesion of WiDr-P and WiDr-HA to F-2 cells was inhibited by the addition of anti-s-Le(a) antibody (Ab). WiDr-P and WiDr-HA both demonstrated the capacity to metastasize to the liver, by the inoculation of cancer cells to the spleen, while WiDr-LA did not do so. The number of metastatic nodules of WiDr-HA was significantly higher than that of WiDr-P. Treatment with anti-s-Le(a) Ab inhibited the metastasis of WiDr-P and WiDr-HA to the liver, but not with anti-s-Le(x) Ab. CONCLUSIONS: These findings suggest that s-Le(a) plays an important role in cell adhesion molecules, in the metastasis of colon cancer.
BACKGROUND: The vascular endothelium plays a critical role in cancer metastasis by directing circulating cancer cells into extravascular tissues and by expressing cell adhesion molecules. The authors investigated the interaction between a cell line derived from humancolon cancer and cultured murine endothelial cells, in vitro, and in vivo. MATERIALS AND METHODS: Variant cell lines with high (WiDr-HA) or low (WiDr-LA) levels of cell surface sialyl Lewis(a) antigen (s-Le(a)) were isolated from the heterogeneous WiDr cells (WiDr-P) in order to characterize the biological behavior of colon cancer cells having elevated cell surface contents of s-Le(a). RESULTS: A correlation was found to exist between the degree of s-Le(a) expression, and the attachment of cancer cells to activated human umbilical vein endothelial cells, or to F-2 cells isolated from murine vascular endothelial cells. The adhesion of WiDr-P and WiDr-HA to F-2 cells was inhibited by the addition of anti-s-Le(a) antibody (Ab). WiDr-P and WiDr-HA both demonstrated the capacity to metastasize to the liver, by the inoculation of cancer cells to the spleen, while WiDr-LA did not do so. The number of metastatic nodules of WiDr-HA was significantly higher than that of WiDr-P. Treatment with anti-s-Le(a) Ab inhibited the metastasis of WiDr-P and WiDr-HA to the liver, but not with anti-s-Le(x) Ab. CONCLUSIONS: These findings suggest that s-Le(a) plays an important role in cell adhesion molecules, in the metastasis of colon cancer.
Authors: Ritsuko Sawada; Shu-Man Sun; Xiaohong Wu; Feng Hong; Govind Ragupathi; Philip O Livingston; Wolfgang W Scholz Journal: Clin Cancer Res Date: 2011-02-22 Impact factor: 12.531
Authors: A Opolski; A Laskowska; J Madej; J Wietrzyk; A Kłopocki; C Radzikowski; M Ugorski Journal: Clin Exp Metastasis Date: 1998-11 Impact factor: 5.150
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Authors: Susan N Thomas; Fei Zhu; Ronald L Schnaar; Christina S Alves; Konstantinos Konstantopoulos Journal: J Biol Chem Date: 2008-03-27 Impact factor: 5.157
Authors: Shreya Gupta; James D McDonald; Reed I Ayabe; Tahsin M Khan; Lauren A Gamble; Surajit Sinha; Cathleen Hannah; Andrew M Blakely; Jeremy L Davis; Jonathan M Hernandez Journal: J Gastrointest Oncol Date: 2020-04