| Literature DB >> 941195 |
E D Helton, M C Williams, J W Goldzieher.
Abstract
Chromatographic profiles of the conjugates of 17alpha-ethynylestradiol (EE2) were obtained from the urine of castrate and normal women given tritium labeled EE2 orally. Five distinct radioactive peaks were observed, with considerable quantitative variation between individuals. Glucosiduronate fractions comprised the dominant excreted radioactivity. In vitro incubation of normal human liver also produced five conjugate types. Simultaneous intravenous/oral (14C/3H) administration of EE2 demonstrated conversion to identical urinary conjugate and free steroid products, with a greater excretion of the intravenous material. Authentic 3-glucuronide of EE2 was synthesized and its position in the chromatographic system relative to the urinary conjugate peaks demonstrated.Entities:
Keywords: Biology; Castration; Clinical Research; Contraception; Contraceptive Agents, Estrogen--administraction and dosage; Contraceptive Agents, Estrogen--analysis; Contraceptive Agents, Estrogen--side effects; Contraceptive Agents, Female--administraction and dosage; Contraceptive Agents, Female--analysis; Contraceptive Agents, Female--side effects; Contraceptive Agents--administraction and dosage; Contraceptive Agents--analysis; Contraceptive Agents--side effects; Enzymatic Effects; Enzymes And Enzyme Inhibitors; Estrogens; Ethinyl Estradiol--administraction and dosage; Ethinyl Estradiol--analysis; Ethinyl Estradiol--side effects; Examinations And Diagnoses; Family Planning; Hepatic Effects; Hormones; Human Volunteers; Hysterectomy; In Vitro; Laboratory Examinations And Diagnoses; Laboratory Procedures; Metabolic Effects; Physiology; Research Methodology; Steroid Metabolic Effects; Women
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Year: 1976 PMID： 941195 DOI： 10.1016/0039-128x(76)90144-6
Source DB: PubMed Journal: Steroids ISSN： 0039-128X Impact factor: 2.668