Cloning of the murine tetranectin gene and 5'-flanking region.

The gene encoding murine tetranectin (Tna) and its 5'-flanking region was isolated and cloned from a EMBL3 SP6/T7 genomic library. The compiled nucleotide sequence was determined by sequencing, revealing a conserved Tna structure in man and mouse. Mapping of the transcription start point (tsp) suggests that murine Tna has more than one of these. In addition, no consensus TATA-box was found uptream for the putative tsp(s) in the 5'-flanking region of the gene, indicating that the murine Tna promoter belongs to the TATA-less class of genes. The cloned murine Tna was mapped to region F1-F3 on mouse chromosome 9 by fluorescence in situ hybridization (FISH).