The influence of hyperbaric oxygenation on leukocyte viability and surface protein expression.

Hyperbaric oxygenation was studied as a potential inducer of cell growth and differentiation in promyelocytic leukemic HL60 cells. We studied changes in HL60 cells exposed to hyperbaric oxygen, oxygen, or carbon dioxide for 72 h. The proliferation rate and viability of cells in the hyperbaric oxygenation groups were significantly lower (p < 0.05) than for the controls. While CD13 and CD38 were expressed less following hyperbaric treatment, CD11b, CD14, and CD16 showed an increase following hyperbaric treatment, and CD10, CD15, and HLADR showed no change. These results support previous studies which demonstrate the role of oxygen tension in the regulation of cell cycle and protein expression.