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Literature DB >> 940774

Elimination of double strand nuclease activity from S1 nuclease prepared from crude alpha amylase.

Abstract

Single strand-specific s1 nuclease prepared as previously described from crude alpha amylase by DEAE-cellulose chromatography also contains nuclease which degrades double strand nucleic acid. The double strand activity can be removed by repeating the DEAE-cellulose chromatography procedure at least two additional times. S1 nuclease prepared by this procedure does not degrade double strand sheared DNA as measured by Sephadex chromatography. Under the same conditions single strand DNA is completely degraded. Thus, S1 nuclease prepared by this procedure is suitable for use in removing single strand regions in DNA/DNA duplexes and DNA/RNA hybrids.

Entities: Chemical

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Year: 1976 PMID： 940774 PMCID： PMC342995 DOI： 10.1093/nar/3.5.1419

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

9 in total

1. AN ENDONUCLEASE FROM NEUROSPORA CRASSA SPECIFIC FOR POLYNUCLEOTIDES LACKING AN ORDERED STRUCTURE. I. PURIFICATION AND PROPERTIES OF THE ENZYME.

Authors: S LINN; I R LEHMAN
Journal: J Biol Chem Date: 1965-03 Impact factor: 5.157

6. Mung bean nuclease I. II. Resistance of double stranded deoxyribonucleic acid and susceptibility of regions rich in adenosine and thymidine to enzymatic hydrolysis.

Authors: P H Johnson; M Laskowski
Journal: J Biol Chem Date: 1970-02-25 Impact factor: 5.157

1 in total

1. Assay of DNA-RNA hybrids by S1 nuclease digestion and adsorption to DEAE-cellulose filters.

Authors: I H Maxwell; J Van Ness; W E Hahn
Journal: Nucleic Acids Res Date: 1978-06 Impact factor: 16.971

1 in total

Elimination of double strand nuclease activity from S1 nuclease prepared from crude alpha amylase.

1. AN ENDONUCLEASE FROM NEUROSPORA CRASSA SPECIFIC FOR POLYNUCLEOTIDES LACKING AN ORDERED STRUCTURE. I. PURIFICATION AND PROPERTIES OF THE ENZYME.

2. CHARACTERIZATION OF A DEOXYRIBONUCLEASE OF MUSTELUS CANIS LIVER.

3. Purification of S1 muclease from Takadiastase by affinity chromatography on single-stranded DNA-acrylamide columns.

4. Determination of protein: a modification of the Lowry method that gives a linear photometric response.

5. A nuclease specific for heat-denatured DNA in isolated from a product of Aspergillus oryzae.

6. Mung bean nuclease I. II. Resistance of double stranded deoxyribonucleic acid and susceptibility of regions rich in adenosine and thymidine to enzymatic hydrolysis.

7. Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.

8. A crude nuclease preparation suitable for use in DNA reassociation experiments.

9. Specific inhibition by ATP and other properites of an endonuclease of Neurospora crassa.

1. Assay of DNA-RNA hybrids by S1 nuclease digestion and adsorption to DEAE-cellulose filters.