Literature DB >> 9406186

Enzymatic cleavage and HPLC peptide mapping of proteins.

K R Williams1, K L Stone.   

Abstract

Detailed procedures are described for successfully digesting reasonably small quantities (i.e., usually > 10 pmol) of proteins with a variety of proteases and for then isolating the resulting peptides by reverse-phase HPLC. Since sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) appears to be the current method of choice for final purification of proteins for structural analysis, special attention is given to carrying out in-gel proteolytic digests on SDS-PAGE-separated proteins that have usually been stained with Coomassie Blue. A compilation of data from nearly 200 "unknown" samples is used to help provide realistic expectations with respect to the results that are likely to be obtained from carrying out in-gel proteolytic digests on large numbers of proteins.

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Year:  1997        PMID： 9406186     DOI： 10.1007/BF02752260

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  10 in total

1.  In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis.

Authors:  J Rosenfeld; J Capdevielle; J C Guillemot; P Ferrara
Journal:  Anal Biochem       Date:  1992-05-15       Impact factor: 3.365

2.  Reversed-phase high-performance liquid chromatography for fractionation of enzymatic digests and chemical cleavage products of proteins.

Authors:  K L Stone; J I Elliott; G Peterson; W McMurray; K R Williams
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.

Authors:  R H Aebersold; J Leavitt; R A Saavedra; L E Hood; S B Kent
Journal:  Proc Natl Acad Sci U S A       Date:  1987-10       Impact factor: 11.205

5.  Improvement of an "In-Gel" digestion procedure for the micropreparation of internal protein fragments for amino acid sequencing.

Authors:  U Hellman; C Wernstedt; J Góñez; C H Heldin
Journal:  Anal Biochem       Date:  1995-01-01       Impact factor: 3.365

6.  Internal protein sequence analysis: enzymatic digestion for less than 10 micrograms of protein bound to polyvinylidene difluoride or nitrocellulose membranes.

Authors:  J Fernandez; M DeMott; D Atherton; S M Mische
Journal:  Anal Biochem       Date:  1992-03       Impact factor: 3.365

7.  Production and separation of peptides from proteins stained with Coomassie brilliant blue R-250 after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  H Kawasaki; Y Emori; K Suzuki
Journal:  Anal Biochem       Date:  1990-12       Impact factor: 3.365

8.  A comparison of the binding of Coomassie brilliant blue to proteins at low and neutral pH.

Authors:  H J Chial; A G Splittgerber
Journal:  Anal Biochem       Date:  1993-09       Impact factor: 3.365

9.  Funnel-well SDS-PAGE: a rapid technique for obtaining sufficient quantities of low-abundance proteins for internal sequence analysis.

Authors:  G Lombard-Platet; P Jalinot
Journal:  Biotechniques       Date:  1993-10       Impact factor: 1.993

10.  Assignment of the inter- and intramolecular disulfide linkages in recombinant human macrophage colony stimulating factor using fast atom bombardment mass spectrometry.

Authors:  M O Glocker; B Arbogast; J Schreurs; M L Deinzer
Journal:  Biochemistry       Date:  1993-01-19       Impact factor: 3.162
  10 in total
  10 in total

1.  Toluene diisocyanate reactivity with glutathione across a vapor/liquid interface and subsequent transcarbamoylation of human albumin.

Authors:  Adam V Wisnewski; Justin M Hettick; Paul D Siegel
Journal:  Chem Res Toxicol       Date:  2011-08-10       Impact factor: 3.739

2.  Whey milk proteomics from Schistosoma mansoni-infected mice reveals proteins involved in immunomodulation of the offspring.

Authors:  Gabriela Calixto Ribeiro de Holanda; Iasmim Lopes de Lima; Sandra Mara Naressi Scapin; Maria da Conceição Silva; Iana Rafaela Fernandes Sales; José Mauro Granjeiro; Roberto Afonso da Silva; Valdenia Maria Oliveira de Souza; José Luiz de Lima Filho
Journal:  Parasitol Res       Date:  2020-03-04       Impact factor: 2.289

3.  Purification and characterization of a cellular protein that binds to the downstream activation sequence of the strict late UL38 promoter of herpes simplex virus type 1.

Authors:  M D Petroski; E K Wagner
Journal:  J Virol       Date:  1998-10       Impact factor: 5.103

4.  Connecting glutathione with immune responses to occupational methylene diphenyl diisocyanate exposure.

Authors:  Adam V Wisnewski; Jian Liu; Carrie A Redlich
Journal:  Chem Biol Interact       Date:  2013-06-20       Impact factor: 5.192

5.  Antigenic changes in human albumin caused by reactivity with the occupational allergen diphenylmethane diisocyanate.

Authors:  Adam V Wisnewski; Jian Liu; Carrie A Redlich
Journal:  Anal Biochem       Date:  2010-02-01       Impact factor: 3.365

6.  Proteolysis of apolipoprotein A-I by secretory phospholipase A₂: a new link between inflammation and atherosclerosis.

Authors:  Giorgio Cavigiolio; Shobini Jayaraman
Journal:  J Biol Chem       Date:  2014-02-12       Impact factor: 5.157

7.  Hexamethylene diisocyanate (HDI) vapor reactivity with glutathione and subsequent transfer to human albumin.

Authors:  Adam V Wisnewski; Morgen Mhike; Justin M Hettick; Jian Liu; Paul D Siegel
Journal:  Toxicol In Vitro       Date:  2012-11-23       Impact factor: 3.500

8.  Multisite phosphorylation of Pin1-associated mitotic phosphoproteins revealed by monoclonal antibodies MPM-2 and CC-3.

Authors:  Alexandra L Albert; Sébastien B Lavoie; Michel Vincent
Journal:  BMC Cell Biol       Date:  2004-06-01       Impact factor: 4.241

9.  Purification procedure for the isolation of a P-I metalloprotease and an acidic phospholipase A2 from Bothrops atrox snake venom.

Authors:  Danilo L Menaldo; Anna L Jacob-Ferreira; Carolina P Bernardes; Adélia C O Cintra; Suely V Sampaio
Journal:  J Venom Anim Toxins Incl Trop Dis       Date:  2015-08-13

10.  Borrelia burgdorferi small lipoprotein Lp6.6 is a member of multiple protein complexes in the outer membrane and facilitates pathogen transmission from ticks to mice.

Authors:  Kamoltip Promnares; Manish Kumar; Deborah Y Shroder; Xinyue Zhang; John F Anderson; Utpal Pal
Journal:  Mol Microbiol       Date:  2009-08-24       Impact factor: 3.501
  10 in total

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