Literature DB >> 9406183

An effective method of completely removing contaminating genomic DNA from an RNA sample to be used for PCR.

A Sanyal1, S W O'Driscoll, M E Bolander, G Sarkar.   

Abstract

A simple method for removing contaminating genomic DNA from an RNA preparation is presented. The method involves digestion of the RNA with RNase-free DNase I at room temperature followed by inactivation of the enzyme at 65 degrees C in presence of EDTA. This method produces an RNA sample that is negative for genomic DNA by PCR.

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Year:  1997        PMID： 9406183     DOI： 10.1007/BF02752257

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  5 in total

1.  Access to a messenger RNA sequence or its protein product is not limited by tissue or species specificity.

Authors:  G Sarkar; S S Sommer
Journal:  Science       Date:  1989-04-21       Impact factor: 47.728

2.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

3.  Shotgun DNA sequencing using cloned DNase I-generated fragments.

Authors:  S Anderson
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971

4.  Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction.

Authors:  G Gilliland; S Perrin; K Blanchard; H F Bunn
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205

5.  Quantitation of mRNA by the polymerase chain reaction.

Authors:  A M Wang; M V Doyle; D F Mark
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205
  5 in total
  6 in total

1.  Gene digging. A method for obtaining species-specific sequence based on conserved segments of nucleotides in open reading frames.

Authors:  A Sanyal; S W O'Driscoll; J S Fitzsimmons; M E Bolander; G Sarkar
Journal:  Mol Biotechnol       Date:  1998-12       Impact factor: 2.695

2.  Isolation of a cDNA sequence of rabbit GDF5 (mature form) and pattern of its mRNA expression during periosteal chondrogenesis.

Authors:  A Sanyal; G Sarkar; J S Fitzsimmons; S W O'Driscoll
Journal:  Mol Biotechnol       Date:  2000-11       Impact factor: 2.695

3.  The POLARIS gene of Arabidopsis encodes a predicted peptide required for correct root growth and leaf vascular patterning.

Authors:  Stuart A Casson; Paul M Chilley; Jennifer F Topping; I Marta Evans; Martin A Souter; Keith Lindsey
Journal:  Plant Cell       Date:  2002-08       Impact factor: 11.277

4.  High expression of tumor endothelial marker 7 is associated with metastasis and poor survival of patients with osteogenic sarcoma.

Authors:  Bruno Fuchs; Eric Mahlum; Chandralekha Halder; Avudaippan Maran; Michael Yaszemski; Beata Bode; Mark Bolander; Gobinda Sarkar
Journal:  Gene       Date:  2007-05-13       Impact factor: 3.688

5.  The turnip mutant of Arabidopsis reveals that LEAFY COTYLEDON1 expression mediates the effects of auxin and sugars to promote embryonic cell identity.

Authors:  Stuart A Casson; Keith Lindsey
Journal:  Plant Physiol       Date:  2006-08-25       Impact factor: 8.340

6.  An impedance-based integrated biosensor for suspended DNA characterization.

Authors:  Hanbin Ma; Richard W R Wallbank; Reza Chaji; Jiahao Li; Yuji Suzuki; Chris Jiggins; Arokia Nathan
Journal:  Sci Rep       Date:  2013       Impact factor: 4.379
  6 in total

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