Literature DB >> 9405604

Molecular cloning and functional expression of a human cDNA encoding translation initiation factor 6.

K Si1, J Chaudhuri, J Chevesich, U Maitra.   

Abstract

Eukaryotic translation initiation factor 6 (eIF6) binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. In this paper, we devised a procedure for purifying eIF6 from rabbit reticulocyte lysates and immunochemically characterized the protein by using antibodies isolated from egg yolks of laying hens immunized with rabbit eIF6. By using these monospecific antibodies, a 1.096-kb human cDNA that encodes an eIF6 of 245 amino acids (calculated Mr 26,558) has been cloned and expressed in Escherichia coli. The purified recombinant human protein exhibits biochemical properties that are similar to eIF6 isolated from mammalian cell extracts. Database searches identified amino acid sequences from Saccharomyces cerevisiae, Drosophila, and the nematode Caenorhabditis elegans with significant identity to the deduced amino acid sequence of human eIF6, suggesting the presence of homologues of human eIF6 in these organisms.

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Year:  1997        PMID： 9405604      PMCID： PMC24943          DOI： 10.1073/pnas.94.26.14285

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  22 in total

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Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

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Journal:  J Mol Biol       Date:  1973-04-05       Impact factor: 5.469

Review 4.  Role of subunits in the ribosome cycle.

Authors:  B D Davis
Journal:  Nature       Date:  1971-05-21       Impact factor: 49.962

5.  Characterization of translation initiation factor 5 (eIF5) from Saccharomyces cerevisiae. Functional homology with mammalian eIF5 and the effect of depletion of eIF5 on protein synthesis in vivo and in vitro.

Authors:  T Maiti; U Maitra
Journal:  J Biol Chem       Date:  1997-07-18       Impact factor: 5.157

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Authors:  U Maitra; E A Stringer; A Chaudhuri
Journal:  Annu Rev Biochem       Date:  1982       Impact factor: 23.643

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Authors:  D M Valenzuela; A Chaudhuri; U Maitra
Journal:  J Biol Chem       Date:  1982-07-10       Impact factor: 5.157

8.  Mechanism of action of the wheat germ ribosome dissociation factor: interaction with the 60 S subunit.

Authors:  D W Russell; L L Spremulli
Journal:  Arch Biochem Biophys       Date:  1980-05       Impact factor: 4.013

9.  Purification and characterization of a ribosome dissociation factor (eukaryotic initiation factor 6) from wheat germ.

Authors:  D W Russell; L L Spremulli
Journal:  J Biol Chem       Date:  1979-09-25       Impact factor: 5.157

10.  The mechanism of action of eukaryotic initiation factor 4C in protein synthesis.

Authors:  A Thomas; H Goumans; H O Voorma; R Benne
Journal:  Eur J Biochem       Date:  1980
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  14 in total

1.  Opposing action of casein kinase 1 and calcineurin in nucleo-cytoplasmic shuttling of mammalian translation initiation factor eIF6.

Authors:  Arunima Biswas; Shaeri Mukherjee; Supratik Das; Dennis Shields; Chi Wing Chow; Umadas Maitra
Journal:  J Biol Chem       Date:  2010-11-17       Impact factor: 5.157

2.  The Saccharomyces cerevisiae homologue of mammalian translation initiation factor 6 does not function as a translation initiation factor.

Authors:  K Si; U Maitra
Journal:  Mol Cell Biol       Date:  1999-02       Impact factor: 4.272

3.  Dissociation by Pelota, Hbs1 and ABCE1 of mammalian vacant 80S ribosomes and stalled elongation complexes.

Authors:  Vera P Pisareva; Maxim A Skabkin; Christopher U T Hellen; Tatyana V Pestova; Andrey V Pisarev
Journal:  EMBO J       Date:  2011-03-29       Impact factor: 11.598

4.  Mechanism of cytoplasmic mRNA translation.

Authors:  Karen S Browning; Julia Bailey-Serres
Journal:  Arabidopsis Book       Date:  2015-04-24

5.  The Saccharomyces cerevisiae TIF6 gene encoding translation initiation factor 6 is required for 60S ribosomal subunit biogenesis.

Authors:  U Basu; K Si; J R Warner; U Maitra
Journal:  Mol Cell Biol       Date:  2001-03       Impact factor: 4.272

6.  Regulation of eukaryotic translation initiation factor 6 dynamics through multisite phosphorylation by GSK3.

Authors:  Courtney F Jungers; Jonah M Elliff; Daniela S Masson-Meyers; Christopher J Phiel; Sofia Origanti
Journal:  J Biol Chem       Date:  2020-07-23       Impact factor: 5.157

7.  Eukaryotic initiation factor 6, an evolutionarily conserved regulator of ribosome biogenesis and protein translation.

Authors:  Jianjun Guo; Zhaoqing Jin; Xiaohan Yang; Jian-Feng Li; Jin-Gui Chen
Journal:  Plant Signal Behav       Date:  2011-05-01

8.  Phosphorylation of mammalian eukaryotic translation initiation factor 6 and its Saccharomyces cerevisiae homologue Tif6p: evidence that phosphorylation of Tif6p regulates its nucleocytoplasmic distribution and is required for yeast cell growth.

Authors:  Uttiya Basu; Kausik Si; Haiteng Deng; Umadas Maitra
Journal:  Mol Cell Biol       Date:  2003-09       Impact factor: 4.272

Review 9.  Eukaryotic initiation factor 6 mediates a continuum between 60S ribosome biogenesis and translation.

Authors:  Annarita Miluzio; Anne Beugnet; Viviana Volta; Stefano Biffo
Journal:  EMBO Rep       Date:  2009-04-17       Impact factor: 8.807

10.  The biological roles of ITGB4BP and its potential effect on fibrosis.

Authors:  Jianglin Tan; Gaoxing Luo; Jun Wu
Journal:  Int J Burns Trauma       Date:  2011-09-02
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