OBJECTIVE: To evaluate a polymerase chain reaction assay for identification of Campylobacter fetus and differentiation of the defined subspecies. DESIGN: Characterisation of bacterial strains by traditional phenotyping, polymerase chain reaction, a probabilistic identification scheme and macrorestriction profiling using pulsed field gel electrophoresis. PROCEDURE: The results of identification of 99 bacterial strains as determined by conventional phenotyping or by polymerase chain reaction were compared. Two of these were type strains of C fetus subsp fetus and C fetus subsp venerealis; the remaining strains were field isolates putatively identified as C fetus. In cases where the subspecies identity was disputed, isolates were identified by means of a probabilistic identification scheme and by macrorestriction profiling. RESULTS: The agreement between strain identities initially suggested by traditional phenotypic methods and the PCR assay was found to be 80.8%. The polymerase chain reaction proved to be a reliable technique for the species and subspecies identification of C fetus; equivocal results were obtained in only two instances. Initial misidentifications by conventional phenotyping methods were attributed to methodological differences used in various laboratories. CONCLUSION: Our results indicate that misidentification of C fetus in routine diagnostic laboratories may be relatively common. The PCR assay evaluated gave rapid and reproducible results and is thus a valuable adjunctive method for the identification of C fetus and subsequent subspecies differentiation.
OBJECTIVE: To evaluate a polymerase chain reaction assay for identification of Campylobacter fetus and differentiation of the defined subspecies. DESIGN: Characterisation of bacterial strains by traditional phenotyping, polymerase chain reaction, a probabilistic identification scheme and macrorestriction profiling using pulsed field gel electrophoresis. PROCEDURE: The results of identification of 99 bacterial strains as determined by conventional phenotyping or by polymerase chain reaction were compared. Two of these were type strains of C fetus subsp fetus and C fetus subsp venerealis; the remaining strains were field isolates putatively identified as C fetus. In cases where the subspecies identity was disputed, isolates were identified by means of a probabilistic identification scheme and by macrorestriction profiling. RESULTS: The agreement between strain identities initially suggested by traditional phenotypic methods and the PCR assay was found to be 80.8%. The polymerase chain reaction proved to be a reliable technique for the species and subspecies identification of C fetus; equivocal results were obtained in only two instances. Initial misidentifications by conventional phenotyping methods were attributed to methodological differences used in various laboratories. CONCLUSION: Our results indicate that misidentification of C fetus in routine diagnostic laboratories may be relatively common. The PCR assay evaluated gave rapid and reproducible results and is thus a valuable adjunctive method for the identification of C fetus and subsequent subspecies differentiation.
Authors: Cheryl L Waldner; Sarah Parker; Karen M Gesy; Taryn Waugh; Emily Lanigan; John R Campbell Journal: Can J Vet Res Date: 2017-04 Impact factor: 1.310
Authors: Júnior Mário Baltazar de Oliveira; Gesika Maria da Silva; Antônio Fernando Barbosa Batista Filho; Jonas de Melo Borges; Pollyanne Raysa Fernandes de Oliveira; Daniel Friguglietti Brandespim; Rinaldo Aparecido Mota; José Wilton Pinheiro Journal: Trop Anim Health Prod Date: 2015-01-30 Impact factor: 1.559
Authors: Alvaro García-Guerra; Cheryl L Waldner; Andrea Pellegrino; Nicole Macdonald; Bonnie Chaban; Janet E Hill; Steven H Hendrick Journal: Can J Vet Res Date: 2016-01 Impact factor: 1.310
Authors: Paula M Moolhuijzen; Ala E Lew-Tabor; Bartosz M Wlodek; Fernán G Agüero; Diego J Comerci; Rodolfo A Ugalde; Daniel O Sanchez; Rudi Appels; Matthew Bellgard Journal: BMC Microbiol Date: 2009-05-08 Impact factor: 3.605