Literature DB >> 94044

Detection of herpesvirus type-specific antibody by a micro solid phase radioimmunometric assay.

G R Dreesman, D O Matson, R J Courtney, E Adam, J L Melnick.   

Abstract

A micro solid phase radioimmunometric assay has been developed which measures type-specific antibody in human sera to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The test is performed by coating wells of microtiter plates with purified HSV-specified glycoproteins, VP123 and VP119, derived from HSV-1- and HSV-2-infected cells, respectively. The coated wells are then incubated with diluted unadsorbed sera and subsequently with radioiodinated goat anti-human IgG reagent. Because these major viral envelope glycoproteins contain type-specific antigenic determinants, type-specific antibodies are preferentially detected in our assay.

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Year:  1979        PMID： 94044     DOI： 10.1159/000149077

Source DB:  PubMed          Journal:  Intervirology        ISSN： 0300-5526            Impact factor:   1.763


  6 in total

1.  Herpes simplex virus type-selective enzyme-linked immunosorbent assay with Helix pomatia lectin-purified antigens.

Authors:  B Svennerholm; S Olofsson; S Jeansson; A Vahlne; E Lycke
Journal:  J Clin Microbiol       Date:  1984-02       Impact factor: 5.948

2.  Preparation and characterization of specific antisera to individual glycoprotein antigens comprising the major glycoprotein region of herpes simplex virus type 1.

Authors:  R Eberle; R J Courtney
Journal:  J Virol       Date:  1980-09       Impact factor: 5.103

3.  Confirmation of herpes simplex virus type 2 infections in herpes-like genital lesions by a simple complement-fixation test.

Authors:  M Arsenakis; J T May
Journal:  Br J Vener Dis       Date:  1982-02

4.  Detection of type-specific antibody to herpes simplex virus type 1 by radioimmunoassay with herpes simplex virus type 1 glycoprotein C purified with monoclonal antibody.

Authors:  A M Arvin; C M Koropchak; A S Yeager; L Pereira
Journal:  Infect Immun       Date:  1983-04       Impact factor: 3.441

5.  Determination of herpes simplex virus type-specific antibodies by enzyme-linked immunosorbent assay.

Authors:  R M Coleman; L Pereira; P D Bailey; D Dondero; C Wickliffe; A J Nahmias
Journal:  J Clin Microbiol       Date:  1983-08       Impact factor: 5.948

6.  Assay of type-specific and type-common antibodies to herpes simplex virus types 1 and 2 in human sera.

Authors:  R Eberle; R J Courtney
Journal:  Infect Immun       Date:  1981-03       Impact factor: 3.441
  6 in total

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