| Literature DB >> 9395400 |
M B Yaffe1, M Schutkowski, M Shen, X Z Zhou, P T Stukenberg, J U Rahfeld, J Xu, J Kuang, M W Kirschner, G Fischer, L C Cantley, K P Lu.
Abstract
Pin1 is an essential and conserved mitotic peptidyl-prolyl isomerase (PPIase) that is distinct from members of two other families of conventional PPIases, cyclophilins and FKBPs (FK-506 binding proteins). In response to their phosphorylation during mitosis, Pin1 binds and regulates members of a highly conserved set of proteins that overlaps with antigens recognized by the mitosis-specific monoclonal antibody MPM-2. Pin1 is here shown to be a phosphorylation-dependent PPIase that specifically recognizes the phosphoserine-proline or phosphothreonine-proline bonds present in mitotic phosphoproteins. Both Pin1 and MPM-2 selected similar phosphorylated serine-proline-containing peptides, providing the basis for the specific interaction between Pin1 and MPM-2 antigens. Pin1 preferentially isomerized proline residues preceded by phosphorylated serine or threonine with up to 1300-fold selectivity compared with unphosphorylated peptides. Pin1 may thus regulate mitotic progression by catalyzing sequence-specific and phosphorylation-dependent proline isomerization.Entities:
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Year: 1997 PMID： 9395400 DOI： 10.1126/science.278.5345.1957
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728