Induction of micronuclei in human, goat, rabbit peripheral blood lymphocytes and mouse splenic lymphocytes irradiated in vitro with gamma radiation.

The frequencies of gamma-ray-induced micronuclei (MN) in cytokinesis-blocked (CB) lymphocytes at several doses were measured in three donors of four species (human, goat, rabbit, mouse). Measurements performed after irradiation showed a dose-related increases in MN frequency in each of the donors studied. The relative sensitivity of mouse in spleen lymphocytes (SLs), goat in peripheral blood lymphocytes (PBLs) and rabbit PBLs compared with human PBLs was estimated by best fitting linear-quadratic model based on the radiation-induced MN data over the range from 0 to 400 cGy. In the case of MN frequency with 0.2, the relative sensitivities of mouse SLs, goat PBLs and rabbit PBLs were 1.67, 0.98 and 0.39, respectively. These data indicate that the induction of MN in CB cells following irradiation is similar in human and goat PBLs, and PBLs from rabbit were much less sensitive to the MN induction effects of gamma-radiation than those from human. Compared with the radiation-induced MN formation in the PBLs of human, the SLs of mouse were more radiosensitive.