New packing materials for protein chromatography.

This review describes new packing materials designed for protein chromatography, covering advances in base supports and stationary phases. Base supports are classified according to their chemical composition. Since most separation media are bead shaped, typical procedures used for their preparation are also presented. In order to provide matrices combining improved chemical stability and chromatographic performances, composite materials continue to be developed, including bonded stationary phases, pore composites and mixed carriers. The different approaches to their preparation are described and characteristics that play a major role in the chromatographic process are discussed. Recently introduced materials and some of their applications under non-denaturing conditions in the different chromatographic modes are also presented.