Literature DB >> 9391064

A direct electrode-driven P450 cycle for biocatalysis.

V Reipa1, M P Mayhew, V L Vilker.   

Abstract

The large potential of redox enzymes to carry out formation of high value organic compounds motivates the search for innovative strategies to regenerate the cofactors needed by their biocatalytic cycles. Here, we describe a bioreactor where the reducing power to the cycle is supplied directly to purified cytochrome CYP101 (P450cam; EC 1.14.15.1) through its natural redox partner (putidaredoxin) using an antimony-doped tin oxide working electrode. Required oxygen was produced at a Pt counter electrode by water electrolysis. A continuous catalytic cycle was sustained for more than 5 h and 2,600 enzyme turnovers. The maximum product formation rate was 36 nmol of 5-exo-hydroxycamphor/nmol of CYP101 per min.

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Year:  1997        PMID： 9391064      PMCID： PMC28344          DOI： 10.1073/pnas.94.25.13554

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  13 in total

1.  A thermodynamic model of regulation: modulation of redox equilibria in camphor monoxygenase.

Authors:  S G Sligar; I C Gunsalus
Journal:  Proc Natl Acad Sci U S A       Date:  1976-04       Impact factor: 11.205

2.  Some considerations in spectroelectrochemical evaluation of homogeneous electron transfer involving biological molecules.

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Journal:  Anal Chem       Date:  1975-05       Impact factor: 6.986

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Authors:  C B Brewer; J A Peterson
Journal:  J Biol Chem       Date:  1988-01-15       Impact factor: 5.157

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Authors:  J D Lipscomb; S G Sligar; M J Namtvedt; I C Gunsalus
Journal:  J Biol Chem       Date:  1976-02-25       Impact factor: 5.157

5.  Single turnover studies with oxy-cytochrome P-450cam.

Authors:  C B Brewer; J A Peterson
Journal:  Arch Biochem Biophys       Date:  1986-09       Impact factor: 4.013

6.  Cytochrome P-450cam substrate and effector interactions.

Authors:  I C Gunsalus; J R Meeks; J D Lipscomb
Journal:  Ann N Y Acad Sci       Date:  1973       Impact factor: 5.691

7.  An NMR-derived model for the solution structure of oxidized putidaredoxin, a 2-Fe, 2-S ferredoxin from Pseudomonas.

Authors:  T C Pochapsky; X M Ye; G Ratnaswamy; T A Lyons
Journal:  Biochemistry       Date:  1994-05-31       Impact factor: 3.162

8.  NADH- and oxygen-dependent multiple turnovers of cytochrome P-450-CAM without putidaredoxin and putidaredoxin reductase.

Authors:  K S Eble; J H Dawson
Journal:  Biochemistry       Date:  1984-04-24       Impact factor: 3.162

9.  Regioselectivity in the cytochromes P-450: control by protein constraints and by chemical reactivities.

Authors:  R E White; M B McCarthy; K D Egeberg; S G Sligar
Journal:  Arch Biochem Biophys       Date:  1984-02-01       Impact factor: 4.013

10.  Chemical mechanisms for cytochrome P-450 hydroxylation: evidence for acylation of heme-bound dioxygen.

Authors:  S G Sligar; K A Kennedy; D C Pearson
Journal:  Proc Natl Acad Sci U S A       Date:  1980-03       Impact factor: 11.205
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  7 in total

1.  Electrochemistry of mammalian cytochrome P450 2B4 indicates tunable thermodynamic parameters in surfactant films.

Authors:  Katharine D Hagen; James M Gillan; Sang-Choul Im; Sally Landefeld; Griffin Mead; Megan Hiley; Lucy A Waskell; Michael G Hill; Andrew K Udit
Journal:  J Inorg Biochem       Date:  2013-08-14       Impact factor: 4.155

2.  Photosystem I from plants as a bacterial cytochrome P450 surrogate electron donor: terminal hydroxylation of branched hydrocarbon chains.

Authors:  Kenneth Jensen; Jonathan B Johnston; Paul R Ortiz de Montellano; Birger Lindberg Møller
Journal:  Biotechnol Lett       Date:  2011-10-08       Impact factor: 2.461

3.  Efficient bioelectronic actuation of the natural catalytic pathway of human metabolic cytochrome P450s.

Authors:  Sadagopan Krishnan; Dhanuka Wasalathanthri; Linlin Zhao; John B Schenkman; James F Rusling
Journal:  J Am Chem Soc       Date:  2011-01-07       Impact factor: 15.419

4.  Bioelectronic delivery of electrons to cytochrome P450 enzymes.

Authors:  Sadagopan Krishnan; John B Schenkman; James F Rusling
Journal:  J Phys Chem B       Date:  2011-05-17       Impact factor: 2.991

5.  Electrocatalytic drug metabolism by CYP2C9 bonded to a self-assembled monolayer-modified electrode.

Authors:  Mingli Yang; Jarod L Kabulski; Lance Wollenberg; Xinqi Chen; Murali Subramanian; Timothy S Tracy; David Lederman; Peter M Gannett; Nianqiang Wu
Journal:  Drug Metab Dispos       Date:  2009-01-26       Impact factor: 3.922

6.  Control of electrochemical and ferryloxy formation kinetics of cyt P450s in polyion films by heme iron spin state and secondary structure.

Authors:  Sadagopan Krishnan; Amila Abeykoon; John B Schenkman; James F Rusling
Journal:  J Am Chem Soc       Date:  2009-11-11       Impact factor: 15.419

Review 7.  The Oxygen Dilemma: A Severe Challenge for the Application of Monooxygenases?

Authors:  Dirk Holtmann; Frank Hollmann
Journal:  Chembiochem       Date:  2016-06-30       Impact factor: 3.164
  7 in total

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