Literature DB >> 9389409

Propagation and titration of murine cytomegalovirus in a continuous bone marrow-derived stromal cell line (M2-10B4).

M A Lutarewych1, M R Quirk, B A Kringstad, W Li, C M Verfaillie, M C Jordan.   

Abstract

Murine cytomegalovirus (MCMV) can only be propagated effectively in mouse embryo fibroblast (MEF) cells. We demonstrate that MCMV replicates significantly better in M2-10B4 cells, a continuous line of murine bone marrow stromal cells. M2-10B4 cells were also comparable to MEF cells for detection of small amounts of MCMV reactivating from latently infected spleen explants. M2-10B4 cells will be very useful for studies of MCMV pathogenesis.

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Year:  1997        PMID： 9389409     DOI： 10.1016/s0166-0934(97)00126-2

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  10 in total

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2.  Systemic and mucosal infection program protective memory CD8 T cells in the vaginal mucosa.

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Journal:  J Vis Exp       Date:  2013-07-06       Impact factor: 1.355

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Journal:  J Virol       Date:  2005-03       Impact factor: 5.103

8.  Cytomegalovirus Infection: Mouse Model.

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Journal:  Curr Protoc Immunol       Date:  2018-07-25

9.  Murine cytomegalovirus abortively infects human dendritic cells, leading to expression and presentation of virally vectored genes.

Authors:  Xiuqing Wang; Martin Messerle; Ramil Sapinoro; Kathlyn Santos; Peter K Hocknell; Xia Jin; Stephen Dewhurst
Journal:  J Virol       Date:  2003-07       Impact factor: 5.103

10.  Resolving the titer of murine cytomegalovirus by plaque assay using the M2-10B4 cell line and a low viscosity overlay.

Authors:  Katherine A Zurbach; Toktam Moghbeli; Christopher M Snyder
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  10 in total

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