Literature DB >> 938452

A stopped-flow dual-wavelength spectrophotometer suitable for the study of respiratory chains.

P B Garland, S J Littleford, B A Haddock.   

Abstract

The requirements for a dual-wavelength stopped-flow spectrophotometer to be suitable for studying limited quantities of respiratory-chain preparations are described. They can be met by a design using mainly commercially available components. The constructed apparatus has a dead-time of approx. 2.6 ms, a mixing ratio of 17:1, and a minimal requirement for 0.5 ml of mixed reactants per flow.

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Year:  1976        PMID： 938452      PMCID： PMC1172708          DOI： 10.1042/bj1540277

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  2 in total

1.  Kinetic characterization of the membrane-bound cytochromes of Escherichia coli grown under a variety of conditions by using a stopped-flow dual-wavelength spectrophotometer.

Authors:  B A Haddock; J A Downie; P B Garland
Journal:  Biochem J       Date:  1976-02-15       Impact factor: 3.857

2.  High speed optical stopped-flow apparatus.

Authors:  K L Berger; B Balko; W Borcherdt; W Friauff
Journal:  Rev Sci Instrum       Date:  1968-04       Impact factor: 1.523
  2 in total
  3 in total

1.  Kinetic characterization of the membrane-bound cytochromes of Escherichia coli grown under a variety of conditions by using a stopped-flow dual-wavelength spectrophotometer.

Authors:  B A Haddock; J A Downie; P B Garland
Journal:  Biochem J       Date:  1976-02-15       Impact factor: 3.857

2.  Sequence of b cytochromes relative to ubiquinone in the electron transport chain of Escherichia coli.

Authors:  J A Downie; G B Cox
Journal:  J Bacteriol       Date:  1978-02       Impact factor: 3.490

3.  Three functionally different cytochrome b redox centres in pigeon heart mitochondria.

Authors:  U F Rasmussen; H N Rasmussen; B M Jørgensen
Journal:  Biochem J       Date:  1982-02-01       Impact factor: 3.857
  3 in total

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