Literature DB >> 9375788

Cloning and sequence of a type I pullulanase from an extremely thermophilic anaerobic bacterium, Caldicellulosiruptor saccharolyticus.

G D Albertson1, R H McHale, M D Gibbs, P L Bergquist.   

Abstract

A gene coding for a pullulanase from the obligately anaerobic, extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus has been cloned in Escherichia coli. It consists of an open reading frame (pulA) of 2478 bp which codes for an enzyme of 95,732 Da and is flanked by two other open reading frames. A truncated version of the gene which lacks 381 bp of 5'-sequence also has pullulanase activity and it appears that the amino-terminal portion of the gene is not essential for either activity or thermostability. Amino acid sequence comparisons with other published amylases and pullulanases showed that it possesses homology to the four key regions common to these enzymes.

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Year:  1997        PMID： 9375788     DOI： 10.1016/s0167-4781(97)00123-1

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  10 in total

1.  Antigenicity, expression, and molecular characterization of surface-located pullulanase of Streptococcus pneumoniae.

Authors:  R J Bongaerts; H P Heinz; U Hadding; G Zysk
Journal:  Infect Immun       Date:  2000-12       Impact factor: 3.441

2.  Pullulanase type I from Fervidobacterium pennavorans Ven5: cloning, sequencing, and expression of the gene and biochemical characterization of the recombinant enzyme.

Authors:  C Bertoldo; F Duffner; P L Jorgensen; G Antranikian
Journal:  Appl Environ Microbiol       Date:  1999-05       Impact factor: 4.792

3.  Purification and characterization of a cold-adapted pullulanase from a psychrophilic bacterial isolate.

Authors:  Farah Qoura; Skander Elleuche; Thomas Brueck; Garabed Antranikian
Journal:  Extremophiles       Date:  2014-07-29       Impact factor: 2.395

Review 4.  Structure and function of α-glucan debranching enzymes.

Authors:  Marie Sofie Møller; Anette Henriksen; Birte Svensson
Journal:  Cell Mol Life Sci       Date:  2016-05-02       Impact factor: 9.261

5.  Cloning, sequencing, and characterization of a heat- and alkali-stable type I pullulanase from Anaerobranca gottschalkii.

Authors:  Costanzo Bertoldo; Martin Armbrecht; Fiona Becker; Thomas Schäfer; Garabed Antranikian; Wolfgang Liebl
Journal:  Appl Environ Microbiol       Date:  2004-06       Impact factor: 4.792

6.  Pullulanase: role in starch hydrolysis and potential industrial applications.

Authors:  Siew Ling Hii; Joo Shun Tan; Tau Chuan Ling; Arbakariya Bin Ariff
Journal:  Enzyme Res       Date:  2012-09-06

7.  Genome Wide Re-Annotation of Caldicellulosiruptor saccharolyticus with New Insights into Genes Involved in Biomass Degradation and Hydrogen Production.

Authors:  Nupoor Chowdhary; Ashok Selvaraj; Lakshmi KrishnaKumaar; Gopal Ramesh Kumar
Journal:  PLoS One       Date:  2015-07-21       Impact factor: 3.240

Review 8.  Biohydrogen Production by the Thermophilic Bacterium Caldicellulosiruptor saccharolyticus: Current Status and Perspectives.

Authors:  Abraham A M Bielen; Marcel R A Verhaart; John van der Oost; Servé W M Kengen
Journal:  Life (Basel)       Date:  2013-01-17

9.  A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis.

Authors:  Wei Wei; Jing Ma; Si-Qi Chen; Xiang-Hai Cai; Dong-Zhi Wei
Journal:  BMC Biotechnol       Date:  2015-10-19       Impact factor: 2.563

10.  Identification of potential vaccine candidates against Streptococcus pneumoniae by reverse vaccinology approach.

Authors:  Sandipan Talukdar; Shubhranshu Zutshi; K S Prashanth; Kandarpa K Saikia; Parveen Kumar
Journal:  Appl Biochem Biotechnol       Date:  2014-01-31       Impact factor: 2.926
  10 in total

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