Literature DB >> 9375409

Assessing enzyme substrate specificity using combinatorial libraries and electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry.

M Wigger1, J P Nawrocki, C H Watson, J R Eyler, S A Benner.   

Abstract

A model experiment for the 'on-line' screening of substrate libraries by enzymes using combinatorial libraries in combination with electrospray ionization-Fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometry has been performed. The reaction between the electrophilic substrate 1-chloro-2,4-dinitrobenzene and component of a H-gamma-Glu-Cys-Xxx-OH library, catalyzed by glutathione-S-transferase, has been monitored. It shows the feasibility of 'two-dimensional' screening of substrate libraries by ESI-FTICR mass spectrometry.

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Year:  1997        PMID： 9375409     DOI： 10.1002/(SICI)1097-0231(19971030)11:16<1749::AID-RCM91>3.0.CO;2-G

Source DB:  PubMed          Journal:  Rapid Commun Mass Spectrom        ISSN： 0951-4198            Impact factor:   2.419


  5 in total

1.  Mass spectrometry and immobilized enzymes for the screening of inhibitor libraries.

Authors:  M T Cancilla; M D Leavell; J Chow; J A Leary
Journal:  Proc Natl Acad Sci U S A       Date:  2000-10-24       Impact factor: 11.205

2.  Fourier transform-ion cyclotron resonance mass spectrometric resolution, identification, and screening of non-covalent complexes of Hck Src homology 2 domain receptor and ligands from a 324-member peptide combinatorial library.

Authors:  Maria Wigger; John R Eyler; Steven A Benner; Weiqun Li; Alan G Marshall
Journal:  J Am Soc Mass Spectrom       Date:  2002-10       Impact factor: 3.109

3.  Quantitative Collision Cross-Sections from FTICR Linewidth Measurements: Improvements in Theory and Experiment.

Authors:  Elaura Gustafson; Daniel N Mortensen; David V Dearden
Journal:  J Am Soc Mass Spectrom       Date:  2017-07-21       Impact factor: 3.109

4.  Collision Cross Sections for 20 Protonated Amino Acids: Fourier Transform Ion Cyclotron Resonance and Ion Mobility Results.

Authors:  Chad A Jones; David V Dearden
Journal:  J Am Soc Mass Spectrom       Date:  2016-05-24       Impact factor: 3.109

5.  Development of LC-IMS-CID-TOFMS techniques: analysis of a 256 component tetrapeptide combinatorial library.

Authors:  Amy E Hilderbrand; Sunnie Myung; Catherine A Srebalus Barnes; David E Clemmer
Journal:  J Am Soc Mass Spectrom       Date:  2003-12       Impact factor: 3.109
  5 in total

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