Literature DB >> 9369237

Stoichiometry of 7-ethoxycoumarin metabolism by cytochrome P450 2B1 wild-type and five active-site mutants.

X Fang1, Y Kobayashi, J R Halpert.   

Abstract

Recombinant P450 2B1 wild-type and the active-site mutants I114V, F206L, V363A, V363L, and G478S were purified and studied. The efficiency of coupling of reducing equivalents to 7-hydroxycoumarin formation was decreased for all the mutants except I114V. Uncoupling to H2O was increased for F206L, V363A, and G478S, decreased for V363L, and unchanged for I114V. Uncoupling to H2O2 was increased for V363L and decreased for I114V, F206L, and V363A. The findings from this study provide firm biochemical evidence that residues 206, 363, and 478 comprise part of the substrate binding site of P450 2B1.

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Year:  1997        PMID： 9369237     DOI： 10.1016/s0014-5793(97)01173-3

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  7 in total

1.  Functional importance of a peripheral pocket in mammalian cytochrome P450 2B enzymes.

Authors:  Hyun-Hee Jang; Jingbao Liu; Ga-Young Lee; James R Halpert; P Ross Wilderman
Journal:  Arch Biochem Biophys       Date:  2015-08-28       Impact factor: 4.013

2.  Significant increase in phenacetin oxidation on L382V substitution in human cytochrome P450 1A2.

Authors:  Qingbiao Huang; Grazyna D Szklarz
Journal:  Drug Metab Dispos       Date:  2010-03-24       Impact factor: 3.922

3.  Functional characterisation of an engineered multidomain human P450 2E1 by molecular Lego.

Authors:  Michael Fairhead; Silva Giannini; Elizabeth M J Gillam; Gianfranco Gilardi
Journal:  J Biol Inorg Chem       Date:  2005-11-09       Impact factor: 3.358

4.  Coumarin Derivatives as Substrate Probes of Mammalian Cytochromes P450 2B4 and 2B6: Assessing the Importance of 7-Alkoxy Chain Length, Halogen Substitution, and Non-Active Site Mutations.

Authors:  Jingbao Liu; Manish B Shah; Qinghai Zhang; C David Stout; James R Halpert; P Ross Wilderman
Journal:  Biochemistry       Date:  2016-03-24       Impact factor: 3.162

5.  Rapid and quantitative measurement of metabolic stability without chromatography or mass spectrometry.

Authors:  Matthew J Traylor; Jessica D Ryan; Eric S Arnon; Jonathan S Dordick; Douglas S Clark
Journal:  J Am Chem Soc       Date:  2011-07-27       Impact factor: 15.419

6.  Simultaneous measurement of CYP1A2 activity, regioselectivity, and coupling: Implications for environmental sensitivity of enzyme-substrate binding.

Authors:  Matthew J Traylor; Jack Chai; Douglas S Clark
Journal:  Arch Biochem Biophys       Date:  2010-10-08       Impact factor: 4.013

7.  Structural and thermodynamic consequences of 1-(4-chlorophenyl)imidazole binding to cytochrome P450 2B4.

Authors:  Yonghong Zhao; Ling Sun; B K Muralidhara; Santosh Kumar; Mark A White; C David Stout; James R Halpert
Journal:  Biochemistry       Date:  2007-09-22       Impact factor: 3.162
  7 in total

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