Some chickens which are viraemic with subgroup J avian leukosis virus have antibody-forming cells but no circulating antibody.

New immunoperoxidase-based assays for splenic IgG -antibody-forming cells (AFC) and serum IgG-antibody were used to look for antibody to HPRS-103 in meat-type birds. Meat type birds are known to be less likely to produce neutralising antibody and to be less likely to clear virus from their serum than layer-type birds after infection at hatch. In this work all 12 of the brown leghorn layer-type birds and 5/12 of the line 21 meat-type birds had produced AFC and serum antibody and had cleared serum virus at 63, 82 and 110 days of age. None of the seven viraemic line 21 birds contained serum antibody but three produced AFC. The four viraemic line 21 birds which lacked AFC occurred later in the experiment and had a higher level of virus than the three viraemic line 21 birds which possessed AFC. This suggests that most line 21 birds do not control HPRS-103 and eventually become anergic.