Literature DB >> 9368365

High frequency gene transfer by microprojectile bombardment of intact conidia from the entomopathogenic fungus Paecilomyces fumosoroseus.

C C Barreto1, L C Alves, F J Aragão, E Rech, A Schrank, M H Vainstein.   

Abstract

Two different methods, (i) PEG and (ii) biolistic, were employed to transform protoplasts and conidia of Paecilomyces fumosoroseus using hygromycin resistance as selectable marker. Transformation frequencies varied from 1.9 to 2.5 transformants microgram-1 of DNA by the PEG method, and from 33 to 153 transformants microgram-1 of DNA by the biolistic procedure.

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Year:  1997        PMID： 9368365     DOI： 10.1111/j.1574-6968.1997.tb12711.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  3 in total

1.  Stable transformation of the oomycete, Phytophthora infestans, using microprojectile bombardment.

Authors:  Cristina Cvitanich; Howard S Judelson
Journal:  Curr Genet       Date:  2002-12-19       Impact factor: 3.886

2.  In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNA.

Authors:  Maria Laine P Tinoco; Bárbara B A Dias; Rebeca C Dall'Astta; João A Pamphile; Francisco J L Aragão
Journal:  BMC Biol       Date:  2010-03-31       Impact factor: 7.431

3.  Agrobacterium-mediated insertional mutagenesis (AIM) of the entomopathogenic fungus Beauveria bassiana.

Authors:  Andreas Leclerque; Hong Wan; Anette Abschütz; Siwei Chen; Galina V Mitina; Gisbert Zimmermann; Hans Ulrich Schairer
Journal:  Curr Genet       Date:  2003-11-22       Impact factor: 3.886
  3 in total

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