Literature DB >> 9367359

Hepatitis C virus negative strand RNA is not detected in peripheral blood mononuclear cells and viral sequences are identical to those in serum: a case against extrahepatic replication.

T Laskus1, M Radkowski, L F Wang, J Cianciara, H Vargas, J Rakela.   

Abstract

Peripheral blood mononuclear cells (PBMCs) from 27 hepatitis C virus (HCV)-infected patients were analysed for the presence of HCV negative strand RNA with strand-specific Tth-based RT-PCR. No negative strand RNA was detected in any sample, and positive strand HCV sequences amplified from PBMCs were identical to those found in serum. These findings suggest that HCV does not replicate in PBMCs, and the presence of HCV sequences at this site is compatible with passive virus adsorption and/or contamination by circulating virus.

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Year:  1997        PMID： 9367359     DOI： 10.1099/0022-1317-78-11-2747

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  19 in total

1.  HCV-RNA positivity in peripheral blood mononuclear cells of patients with chronic HCV infection: does it really mean viral replication?

Authors:  V Meier; S Mihm; P Braun Wietzke; G Ramadori
Journal:  World J Gastroenterol       Date:  2001-04       Impact factor: 5.742

2.  Use of whole blood specimens for routine clinical quantitation of hepatitis C virus RNA does not increase assay sensitivity.

Authors:  L Cook; A M Ross; G B Knight; V Agnello
Journal:  J Clin Microbiol       Date:  2000-12       Impact factor: 5.948

3.  Evidence of distinct populations of hepatitis C virus in the liver and plasma of patients co-infected with HIV and HCV.

Authors:  Jason T Blackard; Gang Ma; Satarupa Sengupta; Christina M Martin; Eleanor A Powell; M Tarek Shata; Kenneth E Sherman
Journal:  J Med Virol       Date:  2014-04-30       Impact factor: 2.327

4.  Viral RNA but no evidence of replication can be detected in the peripheral blood mononuclear cells of hepatitis E virus-infected patients.

Authors:  S K Ippagunta; S Naik; S Jameel; K N S Ramana; R Aggarwal
Journal:  J Viral Hepat       Date:  2010-07-26       Impact factor: 3.728

5.  Hepatitis C virus and peripheral blood mononuclear cell reservoirs Patricia Baré.

Authors:  Patricia Baré
Journal:  World J Hepatol       Date:  2009-10-31

6.  Prospective comparison of whole-blood- and plasma-based hepatitis C virus RNA detection systems: improved detection using whole blood as the source of viral RNA.

Authors:  J T Stapleton; D Klinzman; W N Schmidt; M A Pfaller; P Wu; D R LaBrecque; J q Han; M J Phillips; R Woolson; B Alden
Journal:  J Clin Microbiol       Date:  1999-03       Impact factor: 5.948

7.  Identification of unique hepatitis C virus quasispecies in the central nervous system and comparative analysis of internal translational efficiency of brain, liver, and serum variants.

Authors:  Daniel M Forton; Peter Karayiannis; Nadiya Mahmud; Simon D Taylor-Robinson; Howard C Thomas
Journal:  J Virol       Date:  2004-05       Impact factor: 5.103

8.  Compartmentalization of hepatitis C virus (HCV) during HCV/HIV coinfection.

Authors:  Jason T Blackard; Yoichi Hiasa; Laura Smeaton; Denise J Jamieson; Irma Rodriguez; Kenneth H Mayer; Raymond T Chung
Journal:  J Infect Dis       Date:  2007-05-02       Impact factor: 5.226

9.  Alpha interferon inhibits hepatitis C virus replication in primary human hepatocytes infected in vitro.

Authors:  Valérie Castet; Chantal Fournier; Alexandre Soulier; Rozenn Brillet; Joliette Coste; Dominique Larrey; Daniel Dhumeaux; Patrick Maurel; Jean-Michel Pawlotsky
Journal:  J Virol       Date:  2002-08       Impact factor: 5.103

10.  Intrahepatic hepatitis C virus replication correlates with chronic hepatitis C disease severity in vivo.

Authors:  Sampa Pal; Margaret C Shuhart; Lisa Thomassen; Scott S Emerson; Tao Su; Nathan Feuerborn; John Kae; David R Gretch
Journal:  J Virol       Date:  2006-03       Impact factor: 5.103
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