Literature DB >> 9359116

Recovery of homogeneous and functional beta 2-adrenergic receptors from extracellular baculovirus particles.

T P Loisel1, H Ansanay, S St-Onge, B Gay, P Boulanger, A D Strosberg, S Marullo, M Bouvier.   

Abstract

Expression in baculovirus-infected insect cells allows sufficient production of G-protein coupled receptor for structural studies. An important drawback of this expression system comes from the presence of unprocessed and biologically inactive receptors that have to be eliminated during receptor purification steps. We show that viral particles released from Sf9 cells infected with a recombinant baculovirus coding for the human beta 2-adrenergic receptor (beta 2AR) cDNA contain glycosylated and biologically active beta 2AR. In addition, post-translational modifications known to modulate receptor activity were found to occur in these particles.

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Year:  1997        PMID： 9359116     DOI： 10.1038/nbt1197-1300

Source DB:  PubMed          Journal:  Nat Biotechnol        ISSN： 1087-0156            Impact factor:   54.908


  11 in total

Review 1.  Therapeutic antibodies directed at G protein-coupled receptors.

Authors:  Catherine J Hutchings; Markus Koglin; Fiona H Marshall
Journal:  MAbs       Date:  2010-11-01       Impact factor: 5.857

Review 2.  Opportunities for therapeutic antibodies directed at G-protein-coupled receptors.

Authors:  Catherine J Hutchings; Markus Koglin; William C Olson; Fiona H Marshall
Journal:  Nat Rev Drug Discov       Date:  2017-07-14       Impact factor: 84.694

3.  Adenovirus mediated expression "in vivo" of the chemokine receptor CXCR1.

Authors:  J Sarmiento; K E Kypreos; G N Prado; K Suetomi; C Stanzel; C Maxwell; D Shumate; M R Tandang-Silvas; K Rajarathnam; J Navarro
Journal:  J Struct Funct Genomics       Date:  2008-12-04

4.  Improved adenovirus type 5 vector-mediated transduction of resistant cells by piggybacking on coxsackie B-adenovirus receptor-pseudotyped baculovirus.

Authors:  Ophélia Granio; Marine Porcherot; Stéphanie Corjon; Kuntida Kitidee; Petra Henning; Assia Eljaafari; Andrea Cimarelli; Leif Lindholm; Pierre Miossec; Pierre Boulanger; Saw-See Hong
Journal:  J Virol       Date:  2009-04-08       Impact factor: 5.103

Review 5.  Baculovirus as versatile vectors for protein expression in insect and mammalian cells.

Authors:  Thomas A Kost; J Patrick Condreay; Donald L Jarvis
Journal:  Nat Biotechnol       Date:  2005-05       Impact factor: 54.908

6.  A novel screening system for claudin binder using baculoviral display.

Authors:  Hideki Kakutani; Azusa Takahashi; Masuo Kondoh; Yumiko Saito; Toshiaki Yamaura; Toshiko Sakihama; Takao Hamakubo; Kiyohito Yagi
Journal:  PLoS One       Date:  2011-02-14       Impact factor: 3.240

7.  Baculovirus display of single chain antibody (scFv) using a novel signal peptide.

Authors:  Kuntida Kitidee; Sawitree Nangola; Gaëlle Gonzalez; Pierre Boulanger; Chatchai Tayapiwatana; Saw-See Hong
Journal:  BMC Biotechnol       Date:  2010-11-19       Impact factor: 2.563

8.  Purification of functional baculovirus particles from silkworm larval hemolymph and their use as nanoparticles for the detection of human prorenin receptor (PRR) binding.

Authors:  Tatsuya Kato; Fumiaki Suzuki; Enoch Y Park
Journal:  BMC Biotechnol       Date:  2011-06-02       Impact factor: 2.563

9.  A simple detection method for low-affinity membrane protein interactions by baculoviral display.

Authors:  Toshiko Sakihama; Takato Sato; Hiroko Iwanari; Toshio Kitamura; Shimon Sakaguchi; Tatsuhiko Kodama; Takao Hamakubo
Journal:  PLoS One       Date:  2008-12-24       Impact factor: 3.240

10.  High-titer preparation of Bombyx mori nucleopolyhedrovirus (BmNPV) displaying recombinant protein in silkworm larvae by size exclusion chromatography and its characterization.

Authors:  Tatsuya Kato; Suganthi Lavender Manoha; Shigeyasu Tanaka; Enoch Y Park
Journal:  BMC Biotechnol       Date:  2009-06-12       Impact factor: 2.563
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