Literature DB >> 9356294

Crystallization and characterization of bovine liver glutamate dehydrogenase.

P E Peterson1, J Pierce, T J Smith.   

Abstract

Bovine liver glutamate dehydrogenase has been crystallized as an abortive complex with glutamic acid, NADH, and an inhibitor, GTP. Crystals of this complex were grown using the sitting drop vapor diffusion method with PEG 8000 as the precipitant and diffract to better than 2.5 A resolution. The crystals belong to the space group P2(1) with an entire enzyme hexamer in the crystallographic asymmetric unit. Self-rotation and self-Patterson functions clearly define the orientation and position of this hexameric enzyme.

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Year:  1997        PMID: 9356294     DOI: 10.1006/jsbi.1997.3899

Source DB:  PubMed          Journal:  J Struct Biol        ISSN: 1047-8477            Impact factor:   2.867


  2 in total

Review 1.  The structure and allosteric regulation of mammalian glutamate dehydrogenase.

Authors:  Ming Li; Changhong Li; Aron Allen; Charles A Stanley; Thomas J Smith
Journal:  Arch Biochem Biophys       Date:  2011-11-04       Impact factor: 4.013

2.  A computational analysis of the three isoforms of glutamate dehydrogenase reveals structural features of the isoform EC 1.4.1.4 supporting a key role in ammonium assimilation by plants.

Authors:  Emmanuel Jaspard
Journal:  Biol Direct       Date:  2006-12-15       Impact factor: 4.540

  2 in total

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