Literature DB >> 9352943

cis-Acting sequences required for light-responsive expression of the psbDII gene in Synechococcus sp. strain PCC 7942.

S Anandan1, S S Golden.   

Abstract

We analyzed the sequences required for promoter activity and high-light responsiveness of the psbDII gene in the cyanobacterium Synechococcus sp. strain PCC 7942 by using transcriptional fusions to a lacZ reporter gene. The basal promoter drives high constitutive expression, although no canonical -35 element is evident. The smallest fragment that showed clear light-responsive expression extends from -38 to +160, which includes 52 bp of the psbDII open reading frame. Sequences downstream from the promoter, within the untranslated leader region from +11 to +24, were required for high-light induction.

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Year:  1997        PMID： 9352943      PMCID： PMC179622          DOI： 10.1128/jb.179.21.6865-6870.1997

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  20 in total

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5.  Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.

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Review 6.  Mechanism and control of transcription initiation in prokaryotes.

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Journal:  Annu Rev Biochem       Date:  1985       Impact factor: 23.643

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Authors:  S S Golden; G W Stearns
Journal:  Gene       Date:  1988-07-15       Impact factor: 3.688

8.  Genetic engineering of the cyanobacterial chromosome.

Authors:  S S Golden; J Brusslan; R Haselkorn
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

9.  mRNA stability is regulated by a coding-region element and the unique 5' untranslated leader sequences of the three Synechococcus psbA transcripts.

Authors:  R D Kulkarni; S S Golden
Journal:  Mol Microbiol       Date:  1997-06       Impact factor: 3.501

10.  Expression of a family of psbA genes encoding a photosystem II polypeptide in the cyanobacterium Anacystis nidulans R2.

Authors:  S S Golden; J Brusslan; R Haselkorn
Journal:  EMBO J       Date:  1986-11       Impact factor: 11.598
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