Literature DB >> 9343565

Imaging F-actin in fixed glial cells with a combined optical fluorescence/atomic force microscope.

E Henderson1, D S Sakaguchi.   

Abstract

A prototype combined optical fluorescence/atomic force microscope (OFAFM) designed for use in neurobiology and related disciplines has been constructed and used to study filamentous actin (F-actin) and other cellular structures in fixed Xenopus retinal glial cells (XR1 glial cell line). F-actin was readily observed by both fluorescence and AFM. AFM images of nuclei and other cellular structures were also obtained. The OFAFM consists of an AFM with an interferometer detection mechanism mounted on an inverted optical microscope. Integration of optical and scanned probe imaging methods provides a unique and useful approach to studying glial (and other) cell structure and function.

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Year:  1993        PMID： 9343565     DOI： 10.1006/nimg.1993.1007

Source DB:  PubMed          Journal:  Neuroimage        ISSN： 1053-8119            Impact factor:   6.556


  5 in total

1.  Atomic force and total internal reflection fluorescence microscopy for the study of force transmission in endothelial cells.

Authors:  A B Mathur; G A Truskey; W M Reichert
Journal:  Biophys J       Date:  2000-04       Impact factor: 4.033

2.  Measurements of elastic modulus for human anterior lens capsule with atomic force microscopy: the effect of loading force.

Authors:  Konstantinos T Tsaousis; Panagiotis G Karagiannidis; Nikolaos Kopsachilis; Chrysanthos Symeonidis; Ioannis T Tsinopoulos; Varvara Karagkiozaki; Lampros P Lamprogiannis; Stergios Logothetidis
Journal:  Int Ophthalmol       Date:  2013-09-15       Impact factor: 2.031

3.  Novel combination of atomic force microscopy and epifluorescence microscopy for visualization of leaching bacteria on pyrite.

Authors:  Stefanie Mangold; Kerstin Harneit; Thore Rohwerder; Günter Claus; Wolfgang Sand
Journal:  Appl Environ Microbiol       Date:  2007-11-26       Impact factor: 4.792

4.  Brain tumor classification using AFM in combination with data mining techniques.

Authors:  Marlene Huml; René Silye; Gerald Zauner; Stephan Hutterer; Kurt Schilcher
Journal:  Biomed Res Int       Date:  2013-08-25       Impact factor: 3.411

5.  Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy.

Authors:  Vytautas Navikas; Samuel M Leitao; Kristin S Grussmayer; Adrien Descloux; Barney Drake; Klaus Yserentant; Philipp Werther; Dirk-Peter Herten; Richard Wombacher; Aleksandra Radenovic; Georg E Fantner
Journal:  Nat Commun       Date:  2021-07-27       Impact factor: 14.919
  5 in total

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