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Literature DB >> 9342376

Cloning and characterization of the Flavobacterium johnsoniae (Cytophaga johnsonae) gliding motility gene, gldA.

Abstract

The mechanism of bacterial gliding motility (active movement over surfaces without the aid of flagella) is not known. A large number of nonmotile mutants of the gliding bacterium Flavobacterium johnsoniae (Cytophaga johnsonae) have been previously isolated, and genetic techniques to analyze these mutants have recently been developed. We complemented a nonmotile mutant of F. johnsoniae (UW102-09) with a library of wild-type DNA by using the shuttle cosmid pCP17. The complementing plasmid (pCP100) contained an insert of 13 kbp, and restored motility to 4 of 61 independently isolated nonmotile mutants. A 1.3-kbp fragment that encompassed a single ORF, gldA, complemented all four mutants. Disruption of the chromosomal copy of gldA in wild-type F. johnsoniae UW101 eliminated gliding motility. The predicted protein produced by gldA has strong sequence similarity to ATP binding cassette transport proteins.

Entities: Gene Species

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Year: 1997 PMID： 9342376 PMCID： PMC23729 DOI： 10.1073/pnas.94.22.12139

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

27 in total

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Journal: J Bacteriol Date: 1982-07 Impact factor: 3.490

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Journal: J Bacteriol Date: 1984-07 Impact factor: 3.490

10. Outer membrane polysaccharide deficiency in two nongliding mutants of Cytophaga johnsonae.

Authors: W Godchaux; L Gorski; E R Leadbetter
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74 in total

1. Development and use of a gene deletion strategy for Flavobacterium johnsoniae to identify the redundant gliding motility genes remF, remG, remH, and remI.

Authors: Ryan G Rhodes; Halley G Pucker; Mark J McBride
Journal: J Bacteriol Date: 2011-03-18 Impact factor: 3.490

Cloning and characterization of the Flavobacterium johnsoniae (Cytophaga johnsonae) gliding motility gene, gldA.

1. Plasmids of Escherichia coli as cloning vectors.

2. Identification and analysis of the dissimilatory nitrous oxide reduction genes, nosRZDFY, of Rhizobium meliloti.

3. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

4. Genetic and functional evidence that Type IV pili are required for social gliding motility in Myxococcus xanthus.

5. A region of a cyanobacterial genome required for sulfate transport.

6. An ABC transporter is essential for resistance to the antitumor agent mithramycin in the producer Streptomyces argillaceus.

7. Development of techniques to genetically manipulate members of the genera Cytophaga, Flavobacterium, Flexibacter, and Sporocytophaga.

8. Gliding motility of Cytophaga sp. strain U67.

9. Isolation and characterization of nonspreading mutants of the gliding bacterium Cytophaga johnsonae.

10. Outer membrane polysaccharide deficiency in two nongliding mutants of Cytophaga johnsonae.

1. Development and use of a gene deletion strategy for Flavobacterium johnsoniae to identify the redundant gliding motility genes remF, remG, remH, and remI.

2. A mutation in Flavobacterium psychrophilum tlpB inhibits gliding motility and induces biofilm formation.

3. Mutations in Flavobacterium johnsoniae secDF result in defects in gliding motility and chitin utilization.

4. Transposon mutagenesis in a marine synechococcus strain: isolation of swimming motility mutants.

5. Flavobacterium johnsoniae SprA is a cell surface protein involved in gliding motility.

6. SprB is a cell surface component of the Flavobacterium johnsoniae gliding motility machinery.

7. Cell surface filaments of the gliding bacterium Flavobacterium johnsoniae revealed by cryo-electron tomography.

8. Spirosoma daeguensis sp. nov., isolated from beach soil.

9. Flavobacterium johnsoniae GldH is a lipoprotein that is required for gliding motility and chitin utilization.

10. A protein secretion system linked to bacteroidete gliding motility and pathogenesis.