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Literature DB >> 9342340

Mechanical separation of the complementary strands of DNA.

B Essevaz-Roulet¹, U Bockelmann, F Heslot.

Abstract

We describe the mechanical separation of the two complementary strands of a single molecule of bacteriophage lambda DNA. The 3' and 5' extremities on one end of the molecule are pulled progressively apart, and this leads to the opening of the double helix. The typical forces along the opening are in the range of 10-15 pN. The separation force signal is shown to be related to the local GC vs. AT content along the molecule. Variations of this content on a typical scale of 100-500 bases are presently detected.

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Year: 1997 PMID： 9342340 PMCID： PMC23661 DOI： 10.1073/pnas.94.22.11935

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

28 in total

Review 1. Hybridization and renaturation kinetics of nucleic acids.

Authors: J G Wetmur
Journal: Annu Rev Biophys Bioeng Date: 1976

2. Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads.

Authors: S B Smith; L Finzi; C Bustamante
Journal: Science Date: 1992-11-13 Impact factor: 47.728

3. Extraordinarily stable mini-hairpins: electrophoretical and thermal properties of the various sequence variants of d(GCGAAAGC) and their effect on DNA sequencing.

Authors: I Hirao; Y Nishimura; Y Tagawa; K Watanabe; K Miura
Journal: Nucleic Acids Res Date: 1992-08-11 Impact factor: 16.971

4. Transcription against an applied force.

Authors: H Yin; M D Wang; K Svoboda; R Landick; S M Block; J Gelles
Journal: Science Date: 1995-12-08 Impact factor: 47.728

5. Overstretching B-DNA: the elastic response of individual double-stranded and single-stranded DNA molecules.

Authors: S B Smith; Y Cui; C Bustamante
Journal: Science Date: 1996-02-09 Impact factor: 47.728

6. Predicting DNA duplex stability from the base sequence.

Authors: K J Breslauer; R Frank; H Blöcker; L A Marky
Journal: Proc Natl Acad Sci U S A Date: 1986-06 Impact factor: 11.205

7. Relaxation kinetics of dimer formation by self complementary oligonucleotides.

Authors: M E Craig; D M Crothers; P Doty
Journal: J Mol Biol Date: 1971-12-14 Impact factor: 5.469

8. Direct observation of kinesin stepping by optical trapping interferometry.

Authors: K Svoboda; C F Schmidt; B J Schnapp; S M Block
Journal: Nature Date: 1993-10-21 Impact factor: 49.962

9. DNA stretching on functionalized gold surfaces.

Authors: R M Zimmermann; E C Cox
Journal: Nucleic Acids Res Date: 1994-02-11 Impact factor: 16.971

10. Co-operative non-enzymic base recognition. 3. Kinetics of the helix-coil transition of the oligoribouridylic--oligoriboadenylic acid system and of oligoriboadenylic acid alone at acidic pH.

Authors: D Pörschke; M Eigen
Journal: J Mol Biol Date: 1971-12-14 Impact factor: 5.469

97 in total

10. Force spectroscopy with a small dithering of AFM tip: a method of direct and continuous measurement of the spring constant of single molecules and molecular complexes.

Authors: Lilia A Chtcheglova; George T Shubeita; Sergey K Sekatskii; Giovanni Dietler
Journal: Biophys J Date: 2004-02 Impact factor: 4.033

Mechanical separation of the complementary strands of DNA.

Review 1. Hybridization and renaturation kinetics of nucleic acids.

2. Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads.

3. Extraordinarily stable mini-hairpins: electrophoretical and thermal properties of the various sequence variants of d(GCGAAAGC) and their effect on DNA sequencing.

4. Transcription against an applied force.

5. Overstretching B-DNA: the elastic response of individual double-stranded and single-stranded DNA molecules.

6. Predicting DNA duplex stability from the base sequence.

7. Relaxation kinetics of dimer formation by self complementary oligonucleotides.

8. Direct observation of kinesin stepping by optical trapping interferometry.

9. DNA stretching on functionalized gold surfaces.

10. Co-operative non-enzymic base recognition. 3. Kinetics of the helix-coil transition of the oligoribouridylic--oligoriboadenylic acid system and of oligoriboadenylic acid alone at acidic pH.

1. Transport of torsional stress in DNA.

2. Force-induced melting of the DNA double helix 1. Thermodynamic analysis.

3. Effect of pH on the overstretching transition of double-stranded DNA: evidence of force-induced DNA melting.

4. Dynamic force spectroscopy of single DNA molecules.

5. Unraveling proteins: a molecular mechanics study.

6. Force-induced denaturation of RNA.

7. Force and kinetic barriers to unzipping of the DNA double helix.

8. Unzipping DNA with optical tweezers: high sequence sensitivity and force flips.

9. Force measurements on single molecular contacts through evanescent wave microscopy.

10. Force spectroscopy with a small dithering of AFM tip: a method of direct and continuous measurement of the spring constant of single molecules and molecular complexes.